If you are looking for optimized HPLC method to analyze Colistin A check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

HPLC Method for Colistin A on BIST B+ by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Colistin A.

Colistin A is a last-resort antibiotic drug with the chemical formula C₅₃H₁₀₀N₁₆O₁₃. It is commonly used to treat Gram-negative bacterial infection, such as pneumonia. It is considered to be a critically important and essential medicine for humans. It is available as a generic medication. It works as a polycationic peptide with both hydrophilic and lipophilic moieties, which leads to it displacing magnesium and calcium bacterial counter ion in the lipopolysaccharide in the bacterial outer membrane. You can find detailed UV spectra of Colistin A and information about its various lambda maxima by visiting the following link.

Using SIELC’s newly introduced BIST™ method, Colistin A can be retained on a positively-charged anion-exchange BIST B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged Copper and peptide to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Colistin A can be separated, retained, and UV detected at 200 nm

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