Clotrimazole

HPLC Method for Analysis of Antifungal Agents on Primesep B by SIELC Technologies

Separation and Analysis of Antifungal Agents on a Primesep B Column Using Gradient HPLC Method

Antifungal agents are drugs used to treat fungal infections. Depending on their mechanism of action and chemical structure, antifungal agents can be categorized into several classes. Here are some of the main classes and examples of antifungal agents:

1. Fluconazole: A triazole antifungal mainly used for the treatment and prevention of superficial and systemic fungal infections.
2. Ketoconazole: An imidazole antifungal used to treat a wide variety of fungal infections, though its oral use has become less common due to potential side effects. It’s still frequently used topically.
3. Climbazole: An imidazole antifungal primarily used in hair care products to treat dandruff.
4. Clotrimazole: An imidazole antifungal used to treat various fungal infections including vaginal yeast infections, oral thrush, and ringworm.
5. Itraconazole: A triazole antifungal used primarily to treat a variety of systemic fungal infections.
6. Terbinafine: This compound belongs to the allylamine class. It’s mainly used to treat fungal infections of the nails and skin, like athlete’s foot and ringworm.
7. Econazole: An imidazole antifungal used mainly for skin infections such as athlete’s foot and ringworm.
8. Miconazole: An imidazole antifungal with a broad spectrum of activity. It’s used for a variety of skin infections and also as a vaginal cream for yeast infections.
9. Triclosan: This is a broad-spectrum antimicrobial agent. While it has some antifungal activity, it’s more commonly known for its antibacterial properties. Due to concerns regarding its safety and potential contribution to antibiotic resistance, its use in hand soaps and some other personal care products has been phased out in several regions.

Of these, fluconazole, itraconazole, ketoconazole, climbazole, clotrimazole, econazole, and miconazole belong to the azole class, which primarily acts by inhibiting the fungal enzyme lanosterol 14α-demethylase. This enzyme is crucial for ergosterol synthesis, a vital component of fungal cell membranes. Terbinafine, on the other hand, inhibits squalene epoxidase, another enzyme important in ergosterol synthesis. Triclosan works through a different mechanism, targeting bacterial and fungal fatty acid synthesis.

Antifungal Agents can be separated, retained, and analyzed on a Primesep B mix mode phase column using an gradient analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and sulfuric acid as a buffer. This analysis method can be detected in the UV 200 nm.

HPLC Method for Analysis of Antifungal Agents on Newcrom R1 by SIELC Technologies

Separation and Analysis of Antifungal Agents on a Newcrom R1 Reverse Phase Column Using Gradient HPLC Method

Antifungal agents are drugs used to treat fungal infections. Depending on their mechanism of action and chemical structure, antifungal agents can be categorized into several classes. Here are some of the main classes and examples of antifungal agents:

1. Fluconazole: A triazole antifungal mainly used for the treatment and prevention of superficial and systemic fungal infections.
2. Ketoconazole: An imidazole antifungal used to treat a wide variety of fungal infections, though its oral use has become less common due to potential side effects. It’s still frequently used topically.
3. Climbazole: An imidazole antifungal primarily used in hair care products to treat dandruff.
4. Clotrimazole: An imidazole antifungal used to treat various fungal infections including vaginal yeast infections, oral thrush, and ringworm.
5. Itraconazole: A triazole antifungal used primarily to treat a variety of systemic fungal infections.
6. Terbinafine: This compound belongs to the allylamine class. It’s mainly used to treat fungal infections of the nails and skin, like athlete’s foot and ringworm.
7. Econazole: An imidazole antifungal used mainly for skin infections such as athlete’s foot and ringworm.
8. Miconazole: An imidazole antifungal with a broad spectrum of activity. It’s used for a variety of skin infections and also as a vaginal cream for yeast infections.
9. Triclosan: This is a broad-spectrum antimicrobial agent. While it has some antifungal activity, it’s more commonly known for its antibacterial properties. Due to concerns regarding its safety and potential contribution to antibiotic resistance, its use in hand soaps and some other personal care products has been phased out in several regions.

Of these, fluconazole, itraconazole, ketoconazole, climbazole, clotrimazole, econazole, and miconazole belong to the azole class, which primarily acts by inhibiting the fungal enzyme lanosterol 14α-demethylase. This enzyme is crucial for ergosterol synthesis, a vital component of fungal cell membranes. Terbinafine, on the other hand, inhibits squalene epoxidase, another enzyme important in ergosterol synthesis. Triclosan works through a different mechanism, targeting bacterial and fungal fatty acid synthesis.

Antifungal agents can be separated, retained, and analyzed on a Newcrom R1 reverse phase column using an gradient analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and sulfuric acid as a buffer. This analysis method can be detected in the UV 200 nm.

HPLC Method for Analysis of Clotrimazole, Benzyl alcohol, Ethylparaben on Primesep 200 by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of  Clotrimazole, Benzyl alcohol, Ethylparaben

Clotrimazole is an antifungal medication widely used in the treatment of fungal infections.

Description:

• Classification: Clotrimazole belongs to the class of medications called imidazole antifungals.

Uses:

• Topical: It’s commonly used to treat skin infections such as athlete’s foot, jock itch, ringworm, and other fungal skin infections (candidiasis).
• Vaginal: As a vaginal cream or tablet, it’s used to treat vaginal yeast infections.
• Otic (Ear): Clotrimazole solution can be used to treat fungal ear infections.

Mechanism of Action:

• Clotrimazole works by inhibiting the synthesis of ergosterol, a vital component of fungal cell membranes. When ergosterol synthesis is inhibited, it leads to structural and functional impairment of the fungal cell membrane, thereby killing the fungus or inhibiting its growth.

Clotrimazole belongs to the class of chemical compounds known as imidazoles. Specifically, it’s an antifungal compound used to treat fungal infections. Imidazoles work by inhibiting the enzyme lanosterol 14α-demethylase, which is necessary for the synthesis of ergosterol, an essential component of fungal cell membranes. When ergosterol synthesis is disrupted, it leads to alterations in the cell membrane’s permeability and, eventually, fungal cell death.

Other antifungal agents in the imidazole class include miconazole, econazole, and ketoconazole, among others. These are commonly used in various formulations to treat a range of fungal infections, including athlete’s foot, ringworm, and yeast infections.

Clotrimazole can be retained, and analyzed on a Primesep 200 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid as a buffer. This analysis method can be detected in the UV 200 nm.