Triclosan

HPLC Method for Analysis of Triclosan on Newcrom R1 Column by SIELC Technologies

Separation type: Liquid Chromatography Reversed-phase

Triclosan is an antimicrobial and antifungal agent that has been used in a variety of consumer products, including soaps, toothpastes, deodorants, and cosmetics, as well as in medical devices and textiles. Here are some key points about triclosan:

Mechanism of Action: Triclosan works by inhibiting the synthesis of fatty acids in bacteria, which are essential components of the bacterial cell membrane. Specifically, it targets the enzyme enoyl-acyl carrier protein reductase (ENR).

Controversy and Concerns:

Resistance: There have been concerns that widespread use of triclosan may contribute to antibiotic resistance, as bacteria that become resistant to triclosan might also become resistant to certain antibiotics.
Environmental Concerns: Triclosan is persistent in the environment and has been detected in various water sources. It can be transformed into other chemicals, like dioxins, when exposed to sunlight in aquatic environments.
Health Concerns: Some studies have raised questions about potential endocrine-disrupting effects of triclosan, particularly its impact on thyroid function. Additionally, its potential to contribute to allergic responses, such as skin sensitization, has been discussed.

Triclosan can be retained and analyzed on a mixed-mode Newcrom R1 column with a mobile phase consisting of water, Acetonitrile (MeCN), and sulfuric acid. This analytical method can be detected with high resolution and peak symmetry at a wavelength of 200 nm using UV detection

High Performance Liquid Chromatography (HPLC) Method for Analyses of Triclosan on Newcrom R1 Column by SIELC Technologies

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HPLC Method for Analysis of Antifungal Agents on Newcrom R1 by SIELC Technologies

Separation and Analysis of Antifungal Agents on a Newcrom R1 Reverse Phase Column Using Gradient HPLC Method

Antifungal agents are drugs used to treat fungal infections. Depending on their mechanism of action and chemical structure, antifungal agents can be categorized into several classes. Here are some of the main classes and examples of antifungal agents:

1. Fluconazole: A triazole antifungal mainly used for the treatment and prevention of superficial and systemic fungal infections.
2. Ketoconazole: An imidazole antifungal used to treat a wide variety of fungal infections, though its oral use has become less common due to potential side effects. It’s still frequently used topically.
3. Climbazole: An imidazole antifungal primarily used in hair care products to treat dandruff.
4. Clotrimazole: An imidazole antifungal used to treat various fungal infections including vaginal yeast infections, oral thrush, and ringworm.
5. Itraconazole: A triazole antifungal used primarily to treat a variety of systemic fungal infections.
6. Terbinafine: This compound belongs to the allylamine class. It’s mainly used to treat fungal infections of the nails and skin, like athlete’s foot and ringworm.
7. Econazole: An imidazole antifungal used mainly for skin infections such as athlete’s foot and ringworm.
8. Miconazole: An imidazole antifungal with a broad spectrum of activity. It’s used for a variety of skin infections and also as a vaginal cream for yeast infections.
9. Triclosan: This is a broad-spectrum antimicrobial agent. While it has some antifungal activity, it’s more commonly known for its antibacterial properties. Due to concerns regarding its safety and potential contribution to antibiotic resistance, its use in hand soaps and some other personal care products has been phased out in several regions.

Of these, fluconazole, itraconazole, ketoconazole, climbazole, clotrimazole, econazole, and miconazole belong to the azole class, which primarily acts by inhibiting the fungal enzyme lanosterol 14α-demethylase. This enzyme is crucial for ergosterol synthesis, a vital component of fungal cell membranes. Terbinafine, on the other hand, inhibits squalene epoxidase, another enzyme important in ergosterol synthesis. Triclosan works through a different mechanism, targeting bacterial and fungal fatty acid synthesis.

Antifungal agents can be separated, retained, and analyzed on a Newcrom R1 reverse phase column using an gradient analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and sulfuric acid as a buffer. This analysis method can be detected in the UV 200 nm.