| CAS Number | 55268-75-2 |
|---|---|
| Molecular Formula | C16H16N4O8S |
| Molecular Weight | 424.381 |
| InChI Key | JFPVXVDWJQMJEE-QMTHXVAHSA-N |
| LogP | -0.160 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Cefuroxime
January 14, 2026
If you are looking for optimized HPLC method to analyze Cefuroxime check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Analysis of Cefuroxime on Primesep 100 Column
January 27, 2023
HPLC Method for Analysis of Cefuroxime on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Cefuroxime is a cephalosporin antibiotic used to treat a variety of infections including bronchitis, gonorrhea, and Lyme disease. This antibiotic can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 190 and 275 nm.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Cefuroxime
Condition
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 190, 275 nm |
| Peak Retention Time | 4.81 min |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Cefuroxime |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Separation of Cefuroxime on Newcrom R1 HPLC column
February 16, 2018
Cefuroxime can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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