HPLC Method for Analysis of Cefuroxime on Primesep 100 Column

HPLC Method for Analysis of Cefuroxime on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of  Cefuroxime on Primesep 100 by SIELC Technologies
HPLC Method for Analysis of Cefuroxime on Primesep 100 Column by SIELC Technologies

Cefuroxime is a cephalosporin antibiotic with the chemical formula C16H16N4O8S. It is used to treat a variety of infections including bronchitis, gonorrhea, and Lyme disease. You can find detailed UV spectra of Cefuroxime and information about its various lambda maxima by visiting the following link.

Cefuroxime can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 190 and 275 nm.

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 20/80%
BufferH2SO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 190, 275 nm
Peak Retention Time4.81 min

Description

Class of CompoundsDrug
Analyzing CompoundsCefuroxime

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Cefuroxime
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.