HPLC Method for Analysis of Cefuroxime on Primesep 100 Column

HPLC Method for Analysis of Cefuroxime on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of  Cefuroxime on Primesep 100 by SIELC Technologies
HPLC Method for Analysis of Cefuroxime on Primesep 100 Column by SIELC Technologies

Cefuroxime is a cephalosporin antibiotic used to treat a variety of infections including bronchitis, gonorrhea, and Lyme disease. This antibiotic can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 190 and 275 nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Cefuroxime

Condition

ColumnPrimesep 100, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 20/80%
BufferH2SO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 190, 275 nm
Peak Retention Time4.81 min

Description

Class of CompoundsDrug
Analyzing CompoundsCefuroxime

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Cefuroxime
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.