HPLC Method for Analysis of Carnosine (beta-alanyl-L-histidine) on Primesep 100 by SIELC Technologies

Carnosine is a dipeptide molecule with the chemical formula C₉H₁₄N₄O₃. It is synthesized from beta-alanine and histidine. It is a naturally occurring pH buffer. It is found in muscles and brain tissue, but is produced in the liver. Some use it as a nutritional supplement, but it can be used in topical applications to reduce wrinkles.

Carnosine (beta-alanyl-L-histidine) can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Trifluoroacetic acid (TFA). This analytical method can be UV detected at 215 nm with high resolution and peak symmetry.

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HPLC Method for Carnosine (beta-alanyl-L-histidine) on BIST B+ by SIELC Technologies

Carnosine is a dipeptide molecule with the chemical formula C₉H₁₄N₄O₃. It is synthesized from beta-alanine and histidine. It is a naturally occurring pH buffer. It is found in muscles and brain tissue, but is produced in the liver. Some use it as a nutritional supplement, but it can be used in topical applications to reduce wrinkles.

Using SIELC’s newly introduced BIST™ method, Carnosine (beta-alanyl-L-histidine) can be retained on a positively charged, antion-exchange BIST B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively charged peptide to the positively charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Carnosine can be separated, retained, and UV detected at 205 nm.

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