Captopril

Captopril structural formula

CAS Number62571-86-2
Molecular FormulaC9H15NO3S
Molecular Weight217.280
InChI KeyFAKRSMQSSFJEIM-RQJHMYQMSA-N
LogP0.34
Synonyms
  • Captopril
  • 1-[(2S)-2-Methyl-3-sulfanylpropanoyl]-L-proline
  • L-Proline, 1-[(2S)-3-mercapto-2-methyl-1-oxopropyl]-
  • 62571-86-2
  • L-Proline, 1-[(2S)-3-mercapto-2-methyl-1-oxopropyl]-
  • (-)-1-[(2S)-3-Mercapto-2-methyl-1-oxopropyl]-L-proline
  • (-)-Captopril
  • (S)-1-(3-Mercapto-2-methyl-1-oxopropyl)-L-proline
  • 1-((2S)-3-Mercapto-2-methyl-1-oxopropyl)-L-proline
  • Acediur
  • Aceplus
  • Acepress
  • Acepril
  • Alopresin
  • Angiopril
  • Capoten
  • Capotril
  • Captolane
  • Captoril
  • Captotec
  • Cesplon
  • Dilabar
  • Farcopril
  • Garranil
  • Hipertil
  • Hypopress
  • L-Captopril
  • Lopirin
  • L-Proline, 1-(3-mercapto-2-methyl-1-oxopropyl)-, (S)-
  • Novocaptopril
  • S-Captopril
  • Tensiomin
  • Tensobon
  • Tensoprel
  • EINECS 263-607-1
  • Hypertil
  • 1-(D-3-Mercapto-2-methyl-1-oxopropyl)-L-proline (S,S)
  • 1-(3-Mercapto-2-methyl-1-oxopropyl)-L-proline
  • D-3-Mercapto-2-methylpropionylproline
  • 1-((2S)-3-Mercapto-2-methylpropionyl)-L-proline
  • (2S)-1-(3-Mercapto-2-methylpropionyl)-L-proline
  • 3-Mercapto-2-methylpropionyl-proline
  • Tenosbon
  • Asisten
  • Isopresol
  • Captoprilum
  • UNII-9G64RSX1XD
  • (2S)-1-[(2S)-2-methyl-3-sulfanylpropanoyl]pyrrolidine-2-carboxylic acid
  • CP
  • Captopryl
  • D-2-methyl-3-mercaptopropanoyl-L-proline
  • D-3-mercapto-2-methylpropanoyl-L-proline

Applications:

ELSD HPLC Method for Analysis of Captopril on Newcrom B Column

January 22, 2025

HPLC Method for Captopril on Newcrom B by SIELC Technologies

HPLC Method for Analysis of Captopril on Newcrom B Column by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Captopril

Captopril is a widely used angiotensin-converting enzyme (ACE) inhibitor, effective in managing high blood pressure (hypertension), heart failure, and diabetic kidney complications. By relaxing blood vessels and enhancing blood flow, it reduces strain on the heart and promotes cardiovascular health.

Captopril can be retained, and analyzed using the Newcrom B mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is performed using an Evaporative Light Scattering Detector (ELSD).

ColumnNewcrom B, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN 10%
BufferAmmonium Formate pH 3.0 – 20 mM
Flow Rate1 ml/min
DetectionELSD, the nebulizer and evaporator temperatures 40°C,
with a gas flow rate of 1.6 Standard Liters per Minute (SLM)

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsACE Inhibitor
Analyzing CompoundsCaptopril

Application Column

Newcrom B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Captopril
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Captopril on Newcrom R1 HPLC column

February 16, 2018
Separation of Captopril on Newcrom C18 HPLC column

Captopril can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Captopril
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.