Berberine

Berberine structural formula

UV- Vis Absorption Spectrum of Berberine λ max 230, 264, 348, 431

 

CAS Number2086-83-1
Molecular FormulaC20H18NO4
Molecular Weight336.366
InChI KeyYBHILYKTIRIUTE-UHFFFAOYSA-N
LogP0.717
Synonyms
  • Berberine
  • 9,10-Dimethoxy-5,6-dihydro-2H-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium
  • 1,3-Benzodioxolo[5,6-a]benzo[g]quinolizinium, 5,6-dihydro-9,10-dimethoxy-
  • 2086-83-1
  • Benzo[g]-1,3-benzodioxolo[5,6-a]quinolizinium, 5,6-dihydro-9,10-dimethoxy-
  • 5,6-Dihydro-9,10-dimethoxybenzo[g]-1,3-benzodioxolo[5,6-a]quinolizinium
  • Berbericine
  • Berberin
  • berberina
  • Berbinium, 7,8,13,13a-tetradehydro-9,10-dimethoxy-2,3-(methylenedioxy)-
  • Majarine
  • Thalsine
  • Umbellatin
  • Umbellatine
  • BRN 3570374
  • 9,10-Dimethoxy-2,3-(methylenedioxy)-7,8,13,13a-tetrahydroberbinium
  • EINECS 218-229-1
  • UNII-0I8Y3P32UF
  • 16,17-dimethoxy-5,7-dioxa-13l5-azapentacyclo[11.8.0.02,10.04,8.015,20]henicosa-1(13),2,4(8),9,14,16,18,20-octaen-13-ylium
  • 7,8,13,13a-tetradehydro-9,10-dimethoxy-2,3-[methylenebis(oxy)]berbinium
  • 9,10-dimethoxy-2,3-(methylenedioxy)-7,8,13,13a-tetradehydroberbinium
  • Benzo(g)-1,3-benzodioxolo(5,6-a)quinolizinium, 5,6-dihydro-9,10-dimethoxy-
  • Berberal
  • Coptis rhizome
  • berberine

Applications:

Alltesta HPLC Method for Analysis of Berberine on Primesep B

May 30, 2024

Alltesta HPLC Method for Berberine on Primesep B by SIELC Technologies

HPLC Method for Analysis of Berberine on Primesep B Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Berberine

Berberine is a naturally occurring alkaloid found in several plants, including the roots, rhizomes, and stem bark of plants such as Berberis species (barberry), Coptis chinensis (goldthread), and Hydrastis canadensis (goldenseal).

Biological Activities:

  • Medicinal Properties: Berberine has been used in traditional medicine for its various medicinal properties, including antimicrobial, anti-inflammatory, and antioxidant effects.
  • Blood Sugar Regulation: Some studies suggest that berberine may help regulate blood sugar levels and improve insulin sensitivity, making it of interest in diabetes research.
  • Cholesterol Management: It has been investigated for potential benefits in managing cholesterol levels.
  • Anticancer Properties: Berberine has also shown promise in preclinical studies for its potential anticancer properties.

Uses:

  • Berberine supplements are available and marketed for various health purposes.
  • In traditional medicine, berberine-containing plants have been used for treating various conditions.

Berberine can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV at 275 nm

ColumnPrimesep B, 3.2 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 20/80%
BufferH3PO4 -0.1%
Flow Rate0.5 ml/min
DetectionUV 275 nm

Class of Compounds
Corticosteroids
Analyzing CompoundsBerberine

Application Column

Primesep B

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Berberine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC MS Method for Analysis of Berberine on Primesep B Column

January 29, 2024

HPLC Method for Analysis of Berberine on Primesep B by SIELC Technologies

HPLC Method for Analysis of Berberine on Primesep B Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Berberine

Berberine is a naturally occurring alkaloid found in several plants, including the roots, rhizomes, and stem bark of plants such as Berberis species (barberry), Coptis chinensis (goldthread), and Hydrastis canadensis (goldenseal).

Biological Activities:

  • Medicinal Properties: Berberine has been used in traditional medicine for its various medicinal properties, including antimicrobial, anti-inflammatory, and antioxidant effects.
  • Blood Sugar Regulation: Some studies suggest that berberine may help regulate blood sugar levels and improve insulin sensitivity, making it of interest in diabetes research.
  • Cholesterol Management: It has been investigated for potential benefits in managing cholesterol levels.
  • Anticancer Properties: Berberine has also shown promise in preclinical studies for its potential anticancer properties.

Uses:

  • Berberine supplements are available and marketed for various health purposes.
  • In traditional medicine, berberine-containing plants have been used for treating various conditions.

Berberine can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using LC MS 336 + positive mode

ColumnPrimesep B, 3.2 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 10%
BufferAmmonium formate pH 3.0 – 40 mM
Flow Rate0.5 ml/min
DetectionSIM 336+
Samples0.1 mg/mL in MeCN/H2O – 50/50%
Injection volume1 µl
LOD*80 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Alkaloid, Quaternary ammonium
Analyzing CompoundsBerberine

Application Column

Primesep B

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Berberine

Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Berberine on Primesep B Column

January 25, 2024

HPLC Method for Analysis of Berberine on Primesep B by SIELC Technologies

 HPLC Method for Analysis of   Berberine on Primesep B Column
HPLC Method for Analysis of Berberine on Primesep B Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Berberine

Berberine is a naturally occurring alkaloid found in several plants, including the roots, rhizomes, and stem bark of plants such as Berberis species (barberry), Coptis chinensis (goldthread), and Hydrastis canadensis (goldenseal).

Biological Activities:

  • Medicinal Properties: Berberine has been used in traditional medicine for its various medicinal properties, including antimicrobial, anti-inflammatory, and antioxidant effects.
  • Blood Sugar Regulation: Some studies suggest that berberine may help regulate blood sugar levels and improve insulin sensitivity, making it of interest in diabetes research.
  • Cholesterol Management: It has been investigated for potential benefits in managing cholesterol levels.
  • Anticancer Properties: Berberine has also shown promise in preclinical studies for its potential anticancer properties.

Uses:

  • Berberine supplements are available and marketed for various health purposes.
  • In traditional medicine, berberine-containing plants have been used for treating various conditions.

Berberine can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV at 266 nm

ColumnPrimesep B, 3.2 x 100 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN – 5-20%, 10 min
BufferAmmonium formate pH 3.0 – 40 mM
Flow Rate0.5 ml/min
Detectionλmax230, 266, 348, 431 nm
Samples0.1 mg/mL in MeCN/H2O – 50/50%
Injection volume1 µl
LOD*2 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Alkaloid, Quaternary ammonium
Analyzing CompoundsBerberine

Application Column

Primesep B

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Berberine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Berberine on Newcrom R1 HPLC column

May 16, 2018
Separation of Berberine on Newcrom R1 HPLC column

Berberine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Berberine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.