HPLC Method for Analysis of Berberine on Primesep B Column

HPLC Method for Analysis of Berberine on Primesep B by SIELC Technologies

 HPLC Method for Analysis of   Berberine on Primesep B Column
HPLC Method for Analysis of Berberine on Primesep B Column by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Berberine

Berberine is a naturally occurring alkaloid found in several plants, including the roots, rhizomes, and stem bark of plants such as Berberis species (barberry), Coptis chinensis (goldthread), and Hydrastis canadensis (goldenseal).

Biological Activities:

  • Medicinal Properties: Berberine has been used in traditional medicine for its various medicinal properties, including antimicrobial, anti-inflammatory, and antioxidant effects.
  • Blood Sugar Regulation: Some studies suggest that berberine may help regulate blood sugar levels and improve insulin sensitivity, making it of interest in diabetes research.
  • Cholesterol Management: It has been investigated for potential benefits in managing cholesterol levels.
  • Anticancer Properties: Berberine has also shown promise in preclinical studies for its potential anticancer properties.


  • Berberine supplements are available and marketed for various health purposes.
  • In traditional medicine, berberine-containing plants have been used for treating various conditions.

Berberine can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV at 266 nm

ColumnPrimesep B, 3.2 x 100 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN – 5-20%, 10 min
BufferAmmonium formate pH 3.0 – 40 mM
Flow Rate0.5 ml/min
Detectionλmax230, 266, 348, 431 nm
Samples0.1 mg/mL in MeCN/H2O – 50/50%
Injection volume1 µl
LOD*2 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Alkaloid, Quaternary ammonium
Analyzing CompoundsBerberine

Application Column

Primesep B

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.