HPLC Method for Analysis of Berberine on Primesep B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Berberine
Berberine is a naturally occurring alkaloid found in several plants, including the roots, rhizomes, and stem bark of plants such as Berberis species (barberry), Coptis chinensis (goldthread), and Hydrastis canadensis (goldenseal).
Biological Activities:
- Medicinal Properties: Berberine has been used in traditional medicine for its various medicinal properties, including antimicrobial, anti-inflammatory, and antioxidant effects.
- Blood Sugar Regulation: Some studies suggest that berberine may help regulate blood sugar levels and improve insulin sensitivity, making it of interest in diabetes research.
- Cholesterol Management: It has been investigated for potential benefits in managing cholesterol levels.
- Anticancer Properties: Berberine has also shown promise in preclinical studies for its potential anticancer properties.
Uses:
- Berberine supplements are available and marketed for various health purposes.
- In traditional medicine, berberine-containing plants have been used for treating various conditions.
Berberine can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV at 266 nm
| Column | Primesep B, 3.2 x 100 mm, 5 µm, 100 A |
| Mobile Phase | Gradient MeCN – 5-20%, 10 min |
| Buffer | Ammonium formate pH 3.0 – 40 mM |
| Flow Rate | 0.5 ml/min |
| Detection | λmax230, 266, 348, 431 nm |
| Samples | 0.1 mg/mL in MeCN/H2O – 50/50% |
| Injection volume | 1 µl |
| LOD* | 2 ppb |
| Class of Compounds | Alkaloid, Quaternary ammonium |
| Analyzing Compounds | Berberine |
Application Column
Primesep B
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
BerberineApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
