Benzethonium chloride

Benzethonium chloride structural formula

CAS Number121-54-0
Molecular FormulaC27H42ClNO2
Molecular Weight448.090
InChI KeyUREZNYTWGJKWBI-UHFFFAOYSA-M
LogP3.48
Synonyms
  • Benzethonium chloride
  • N-Benzyl-N,N-dimethyl-2-{2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethoxy}ethan-1-aminium chloride
  • Benzenemethanaminium, N,N-dimethyl-N-[2-[2-[4-(1,1,3,3-tetramethylbutyl)phenoxy]ethoxy]ethyl]-, chloride (1:1)
  • 121-54-0
  • Benzenemethanaminium, N,N-dimethyl-N-(2-(2-(4-(1,1 ,3,3-tetramethylbutyl)phenoxy)ethoxy)ethyl)-, chloride
  • Benzenemethanaminium, N,N-dimethyl-N-[2-[2-[4-(1,1,3,3-tetramethylbutyl)phenoxy]ethoxy]ethyl]-, chloride
  • (Diisobutylphenoxyethoxyethyl)dimethylbenzylammonium chloride
  • [2-[2-(4-Diisobutylphenoxy)ethoxy]ethyl]dimethylbenzylammonium chloride
  • Ammonium, benzyldimethyl[2-[2-[p-(1,1,3,3-tetramethylbutyl)phenoxy]ethoxy]ethyl]-, chloride
  • Anti-Germ 77
  • Antiseptol
  • Bencetonium chloride
  • Benzenemethaminium, N,N-dimethyl-N-[2-[4-(1,1,3,3-tetramethylbutyl)phenoxy]ethoxy]ethyl]-, chloride
  • Benzenemethanaminium, N,N-dimethyl-N-[2-[2-[4-(1,1,3,3-tetramethylbutyl)phenoxy]ethoxy]ethyl]-, chloride (1:1)
  • Benzethionium chloride
  • Benzethoniumchlorid
  • Benzetonium chloride
  • Benzyldimethyl(2-(2-(p-1,1,3,3-tetramethylbutylphenoxy)ethoxy)ethyl)ammonium chloride
  • Benzyldimethyl[2-[2-[4-(1,1,3,3-tetramethylbutyl)phenoxy]ethoxy]ethyl]ammonium chloride
  • Benzyldimethyl[2-[2-[p-(1,1,3,3-tetramethylbutyl)phenoxy]ethoxy]ethyl]ammonium chloride
  • Chlorure de benzethonium
  • cloruro de bencetonio
  • HYAMINE 1622
  • LonzaGuard
  • N,N-Dimethyl-N-(2-(2-(4-(1,1,3,3-tetramethylbutyl)phenoxy)ethoxy)ethyl) benzenemethanaminium chloride
  • N,N-Dimethyl-N-[2-[2-[4-(1,1,3,3-tetramethylbutyl)phenoxy]ethoxy]ethyl]benzenemethanaminium chloride
  • N-Benzyl-N,N-dimethyl-N-(4-[1,1,3,3-tetramethylbutyl]phenoxyethoxyethyl)ammonium chloride
  • Phemeride
  • Phemerol
  • Phemerol chloride
  • Phemithyn
  • Polymine D
  • p-tert-Octylphenoxyethoxyethyldimethylbenzylammonium chloride
  • Quatrachlor
  • Solamin
  • Banagerm
  • Benzethoni chloridum
  • Benzethonium chloride 1622
  • Benzethoniumchloride
  • Benzyldimethyl-p-(1,1,3,3-tetramethylbutyl)phenoxyethoxy-ethylammonium chloride
  • Benzyldimethyl(2-(2-(p-(1,1,3,3-tetramethylbutyl)phenoxy)ethoxy)ethyl)ammonium chloride
  • Benzyldimethyl(2-(2-(4-(1,1,3,3-tetramethylbutyl)phenoxy)ethoxy)ethyl)ammonium chloride
  • BZT (VAN)
  • Caswell No. 614B
  • p-Diisobutyl phenoxyethoxyethyl dimethyl benzylammonium chloride
  • (2-(2-(4-Diisobutylphenoxy)ethoxy)ethyl)dimethylbenzylammonium chloride
  • N,N-Dimethyl-N-(2-(2-(4-(1,1,3,3-tetramethylbutyl)phenoxy)ethoxy)ethyl)-benzenemethanaminium chloride
  • Disilyn
  • EINECS 204-479-9
  • EPA Pesticide Chemical Code 069154
  • Formula 144
  • Inactisol
  • Kylacol
  • NCI-C61494
  • NSC 20200
  • Sanizol
  • Solamine
  • Benzethonii chloridum
  • Benzetonio cloruro
  • Cloruro de benzetonio
  • UNII-PH41D05744
  • BZT
  • N,N-dimethyl-N-(2-{2-[4-(1,1,3,3-tetramethylbutyl)phenoxy]ethoxy}ethyl)-benzenemethanaminium chloride
  • benzyldimethyl(2-{2-[4-(1,1,3,3-tetramethylbutyl)phenoxy]ethoxy}ethyl)ammonium chloride
  • benzyldimethyl(2-{2-[p-(1,1,3,3-tetramethylbutyl)phenoxy]ethoxy}ethyl)ammonium chloride
  • benzyldimethyl-p-(1,1,3,3-tetramethylbutyl)phenoxyethoxy-ethylammonium chloride
  • diisobutylphenoxyethoxyethyl dimethyl benzyl ammonium chloride
  • diisobutylphenoxyethoxyethyldimethyl benzyl ammonium chloride
  • p-diisobutyl phenoxyethoxyethyl dimethyl benzylammonium chloride
  • {2-[2-(4-diisobutylphenoxy)ethoxy]ethyl}dimethylbenzylammonium chloride
  • 324034-09-5
  • 39362-38-4

Applications:

HPLC UV Method for Analysis of Benzethonium Chloride on Primesep SB Column

January 13, 2026

HPLC Method for Benzethonium chloride on Primesep SB by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzethonium chloride.

Benzethonium is a synthetic quaternary ammonium salt with the chemical formula C27H42NO2+. It’s primary used as a disinfectant due to it’s antiseptic and antimicrobial properties. Benzethonium chloride, which is the salt form of this compound, in small concentration is used as skin disinfectant as well as an antimicrobial agent in first aid. You can find detailed UV spectra of Benzethonium chloride and information about its various lambda maxima by visiting the following link.

Benzethonium chloride can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) buffer. Detection is performed using UV.

ColumnPrimesep SB, 2.1 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseGradient MeCN/H2O – 30/70% – 80/20%
BufferAmFm pH 3.0- 10 mM
Flow Rate0.2 mL/min
DetectionUV 275 nm
LOD*0.3 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsSurfactant
Analyzing CompoundsBenzethonium chloride

Application Column

Primesep SB

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Benzethonium chloride
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC MS Method for Analysis of Benzethonium Chloride on Primesep SB Column

January 12, 2026

HPLC Method for Benzethonium chloride on Primesep SB by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzethonium chloride.

Benzethonium is a synthetic quaternary ammonium salt with the chemical formula C27H42NO2+. It’s primary used as a disinfectant due to it’s antiseptic and antimicrobial properties. Benzethonium chloride, which is the salt form of this compound, in small concentration is used as skin disinfectant as well as an antimicrobial agent in first aid. You can find detailed UV spectra of Benzethonium chloride and information about its various lambda maxima by visiting the following link.

Benzethonium chloride can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) buffer. Detection is performed using LC MS.

ColumnPrimesep SB, 2.1 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseGradient MeCN/H2O – 30/70% – 80/20%
BufferAmFm pH 3.0- 10 mM
Flow Rate0.2 mL/min
DetectionLC MS
LOD*8 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsSurfactant
Analyzing CompoundsBenzethonium chloride

Application Column

Primesep SB

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Benzethonium chloride
Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Uv-Vis Spectrum of Benzethonium Chloride

January 9, 2026
Access the UV-Vis Spectrum SIELC Library
UV-Vis Spectrum of Benzethonium chloride.

If you are looking for optimized HPLC method to analyze Benzethonium chloride check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Application Analytes:
Benzethonium chloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Benzethonium chloride on Newcrom R1 HPLC column

February 16, 2018
Separation of Benzethonium chloride on Newcrom C18 HPLC column

Benzethonium chloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Benzethonium chloride
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.