HPLC MS Method for Analysis of Benzethonium Chloride on Primesep SB Column

HPLC Method for Benzethonium chloride on Primesep SB by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzethonium chloride.

Benzethonium is a synthetic quaternary ammonium salt with the chemical formula C27H42NO2+. It’s primary used as a disinfectant due to it’s antiseptic and antimicrobial properties. Benzethonium chloride, which is the salt form of this compound, in small concentration is used as skin disinfectant as well as an antimicrobial agent in first aid. You can find detailed UV spectra of Benzethonium chloride and information about its various lambda maxima by visiting the following link.

Benzethonium chloride can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) buffer. Detection is performed using LC MS.

ColumnPrimesep SB, 2.1 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseGradient MeCN/H2O – 30/70% – 80/20%
BufferAmFm pH 3.0- 10 mM
Flow Rate0.2 mL/min
DetectionLC MS
LOD*8 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsSurfactant
Analyzing CompoundsBenzethonium chloride

Application Column

Primesep SB

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Benzethonium chloride
Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.