HPLC Method for Atenolol on Legacy L1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Atenolol.
Atenolol is a prescription beta-blocker with the chemical formula C14H22N2O3. It is used to treat hypertension and high blood pressure. It can also improve survival chances after a heart attack. Off-label, it is also used to treat migraines and prevent anxiety.
Atenolol can be retained and analyzed using the Legacy L1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
| Column | Legacy L1, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN – 85-60%, 10 min 2 min hold |
| Buffer | Gradient AmFm pH 3.0, 10-80 mM, 10 min 2 min hold |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) MS- compatible mobile phase |
| Class of Compounds |
beta-blocker |
| Analyzing Compounds | Atenolol |
Application Column
Legacy L1
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
AtenololApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
