HPLC Method for Nitrofurantoin on Legacy L1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Nitrofurantoin.
Nitrofurantoin is an oral antibiotic with the chemical formula C8H6N4O5. It is used to treat urinary tract infections.
Nitrofurantoin can be retained and analyzed using the Legacy L1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
| Column | Legacy L1, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 43/55% |
| Buffer | NaHPO4 pH3.5 – 2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 233 nm |
| Class of Compounds |
Drug, Acid, Ionizable, Hydrophobic |
| Analyzing Compounds | Nitrofurantoin |
Application Column
Legacy L1
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
NitrofurantoinApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
