| CAS Number | 55-10-7 |
|---|---|
| Molecular Formula | C9H10O5 |
| Molecular Weight | 198.174 |
| InChI Key | CGQCWMIAEPEHNQ-UHFFFAOYSA-N |
| LogP | -0.2 |
| Synonyms |
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Applications:
HPLC Chiral Separation of Vanillylmandelic Acid on Cyralis CM Column
June 19, 2026
HPLC Method for Analysis of Vanillylmandelic Acid on Cyralis CM Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Vanillylmandelic Acid.
Vanillylmandelic acid is a chemical intermediate with the chemical formula C9H10O5. It is primarily used primarily in synthesis of artificial vanilla flavoring. It is also an end-stage metabolite of epinephrine and norepinephrine. You can find detailed UV spectra of Vanillylmandelic Acid and information about its various lambda maxima by visiting the following link.
This application describes a normal-phase chiral HPLC method for the separation of Vanillylmandelic Acid enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis. Additional peaks that are visible on the chromatogram come from impurities and additives.
Vanillylmandelic Acid can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and TFA. Detection is performed using UV.
Condition
| Column | Cyralis CM, 4.6 x 250 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Hexane/IPA/TFA- 70/28.5/1.5% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 280 nm |
| Resolution | 2.51 |
Description
| Class of Compounds | Intermediate |
| Analyzing Compounds | Vanillylmandelic Acid |
Application Column
Cyralis CM
Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Uv-Vis Spectrum of Vanilmandelic Acid
June 9, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Vanillylmandelic Acid check our HPLC Applications library
UV/Vis.: λmax: 222, 237, 280 nm
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The compound was dissolved in ethanol. This analysis was done with the mobile phase of hexane, IPA, and TFA.
HPLC Separation of Vanilmandelic, Hydroxyindoleacetic, and Homovanillic Acids
November 21, 2006
| Column | Newcrom B , 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 275 nm |
Vanilmandelic, hydroxyindoleacetic and homovanillic acids are metabolites of various neurotransmitters that are used in urine tests to measure the levels of those neurotransmitters in the body. They can be baseline separated on a reverse-phase Primesep D HPLC column by varying the buffers and pH of the mobile phase consisting of water and acetonitrile (MeCN, ACN). UV detection at 230nm.
| Column | Primesep D, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | TFA, AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 230 nm |
| Class of Compounds |
Acid, Monocarboxylic acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Vanilmandelic, Hydroxyindoleacetic, Homovanillic Acid |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options
Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsHomovanillic Acid
Vanillylmandelic Acid
