HPLC Method for Benzoyl peroxide, Hydrogen Peroxide on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Benzoyl peroxide.

Hydrogen Peroxide is a chemical compound with the chemical formula H₂O₂. While most assume it to be a colorless liquid, in it’s natural state, it is pale blue.  It’s primary uses are found in bleaching, as a disinfectant, and sewage treatment. On occasion, it is used as acne treatment as well. It is used in a variety of production of organic peroxy compounds and inorganic peroxides.

Benzoic Acid is an organic compound with the chemical formula C₇H₆O₂. It is considered to be the simplest aromatic carboxylic acid. In nature, it can be found in many plants, especially berries. It is used for food preservation of food, as it can inhibit growth of bacteria and mold. While it is safe in food, it can cause irritation to skin, eye, and respiratory systems. You can find detailed UV spectra of Benzoic Acid and information about its various lambda maxima by visiting the following link.

Benzoyl Peroxide is an organic peroxide with the chemical formula C₁₄H₁₀O₄. It is a commonly used as topical treatment for mild acne because of its antibacterial, anti keratolytic, and comedolytic properties. It is converted to benzoic acid in the skin and primarily targets the pilosebaceous units.

Benzoyl Peroxide can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN) and water. The analysis method can be UV detected at 235 nm.

Urea and carbamide are separated by HILIC HPLC on Primesep N column. Both compounds can be monitored at low UV – 200 nm. Method can be used for determination and quantitation of hydrogen peroxide and urea in various matrices.