HPLC Method for Benzoyl peroxide, Hydrogen Peroxide on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzoyl peroxide.
Hydrogen Peroxide is a chemical compound with the chemical formula H2O2. While most assume it to be a colorless liquid, in it’s natural state, it is pale blue. It’s primary uses are found in bleaching, as a disinfectant, and sewage treatment. On occasion, it is used as acne treatment as well. It is used in a variety of production of organic peroxy compounds and inorganic peroxides.
Benzoic Acid is an organic compound with the chemical formula C7H6O2. It is considered to be the simplest aromatic carboxylic acid. In nature, it can be found in many plants, especially berries. It is used for food preservation of food, as it can inhibit growth of bacteria and mold. While it is safe in food, it can cause irritation to skin, eye, and respiratory systems. You can find detailed UV spectra of Benzoic Acid and information about its various lambda maxima by visiting the following link.
Benzoyl Peroxide is an organic peroxide with the chemical formula C14H10O4. It is a commonly used as topical treatment for mild acne because of its antibacterial, anti keratolytic, and comedolytic properties. It is converted to benzoic acid in the skin and primarily targets the pilosebaceous units.
Benzoyl Peroxide can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN) and water. The analysis method can be UV detected at 235 nm.
| Column | Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 80% |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV 235 nm |
| Class of Compounds | Hydrophobic |
| Analyzing Compounds | Benzoyl peroxide, Hydrogen Peroxide |
Application Column
Newcrom R1
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Hydrogen Peroxide
