HPLC Determination of Benzoyl Peroxide on Newcrom R1 Column

Separation type: Liquid Chromatography Reverse Phase.


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High Performance Liquid Chromatography (HPLC) Method for Analysis of  Benzoyl peroxide.

Benzoyl peroxide is a commonly used topical treatment for mild acne medications because of its antibacterial, anti keratolytic, and comedolytic actions. It is converted to benzoic acid in the skin and primarily targets the pilosebaceous units. Benzoyl Peroxide can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN) and water. The analysis method can be UV detected at 260 nm.


Column Newcrom R1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 80%
Buffer No
Flow Rate 1.0 ml/min
Detection 235 nm


Class of Compounds Hydrophobic
Analyzing Compounds Benzoyl peroxide, Benzoic Acid, Hydrogen peroxide


Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Benzoyl peroxide
Hydrogen Peroxide
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.