Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Guanosine Diphosphate Mannose and Guanosine Diphosphate Fucose.
Two nucleotides containing different sugar fragments were separated on Primesep SB mixed-mode HPLC column using LC/MS compatible conditions. Method can be used for analysis and isolation of guanosine diphosphate mannose and guanosine diphosphate fucose. Mechanism of retention is weak reversed-phase and strong anion-exchange. retention is controlled by buffer concentration and buffer pH. Both compounds exhibit strong interaction providing good retention and separation.
| Column | Primesep SB, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | AmFm pH 2.9- 50 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Hydrophilic, Ionizable, Nucleotide sugar |
| Analyzing Compounds | Diphosphate Mannose, Guanosine Diphosphate Fucose |
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsGuanosine Diphosphate Mannose
