Guanosine Diphosphate Mannose

Guanosine Diphosphate Mannose

CAS Number3123-67-9
Molecular FormulaC16H25N5O16P2
Molecular Weight605.343
InChI KeyMVMSCBBUIHUTGJ-GDJBGNAASA-N
LogP-7.1
Synonyms
  • Diphosphate Mannose, Guanosine
  • Diphosphomannose, Guanosine
  • GDP Mannose
  • Guanosine Diphosphate Mannose
  • Guanosine Diphosphomannose
  • Guanosine Pyrophosphate Mannose
  • Mannose, GDP
  • Mannose, Guanosine Diphosphate
  • Mannose, Guanosine Pyrophosphate
  • Pyrophosphate Mannose, Guanosine

Applications:

Separation of Guanosine Diphosphate Mannose and Guanosine Diphosphate Fucose in Mixed-Mode Chromatography

December 5, 2013

Separation type: Liquid Chromatography Mixed-mode




High Performance Liquid Chromatography (HPLC) Method for Analysis of Guanosine Diphosphate Mannose and Guanosine Diphosphate Fucose.
Two nucleotides containing different sugar fragments were separated on Primesep SB mixed-mode HPLC column using LC/MS compatible conditions. Method can be used for analysis and isolation of guanosine diphosphate mannose and guanosine diphosphate fucose. Mechanism of retention is weak reversed-phase and strong anion-exchange. retention is controlled by buffer concentration and buffer pH. Both compounds exhibit strong interaction providing good retention and separation.

Condition
Column Primesep SB, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer AmFm pH 2.9- 50 mM
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description
Class of Compounds
 Hydrophilic, Ionizable,  Nucleotide sugar
Analyzing Compounds Diphosphate Mannose, Guanosine Diphosphate Fucose

 

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Guanosine Diphosphate Fucose
Guanosine Diphosphate Mannose
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.