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For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Phosphoserine on Primesep B by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Phosphoserine

Phosphoserine is a phosphorylated derivative of the amino acid serine, formed when a phosphate group is attached to its hydroxyl group. It plays a key role in cellular signaling and metabolic processes, serving as a precursor for the biosynthesis of important biomolecules like phospholipids, proteins, and nucleotides. Phosphoserine is also involved in protein phosphorylation, a critical post-translational modification regulating enzyme activity and signal transduction pathways.

Phosphoserine can be retained, separated and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and phosphoric acid as a buffer. This method allows for detection using UV 200 nm.