HPLC Method for Analysis of Phosphoserine on Primesep B Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Phosphoserine on Primesep B by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Phosphoserine on Primesep B Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Phosphoserine

Phosphoserine is a phosphorylated derivative of the amino acid serine, formed when a phosphate group is attached to its hydroxyl group. It plays a key role in cellular signaling and metabolic processes, serving as a precursor for the biosynthesis of important biomolecules like phospholipids, proteins, and nucleotides. Phosphoserine is also involved in protein phosphorylation, a critical post-translational modification regulating enzyme activity and signal transduction pathways.

Phosphoserine can be retained, separated and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and phosphoric acid as a buffer. This method allows for detection using UV 200 nm.

ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 10%
BufferH3PO4 – 0.3%
Flow Rate1.0 ml/min
DetectionUV 200
Sample1.0 mg/mL
DiluentH2O- 100%
LOD*5.1 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Amino acid
Analyzing CompoundsPhosphoserine

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Phosphoserine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.