| CAS Number | 150-76-5 |
|---|---|
| Molecular Formula | C7H8O2 |
| Molecular Weight | 124.139 |
| InChI Key | NWVVVBRKAWDGAB-UHFFFAOYSA-N |
| LogP | 1.58 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Hydroquinone and 4-Methoxyphenol (Mequinol) on Primesep 100 Column
August 15, 2024
High Performance Liquid Chromatography (HPLC) Method for Analysis of Hydroquinone, 4-Methoxyphenol on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Hydroquinone, 4-Methoxyphenol
Hydroquinone (C₆H₄(OH)₂) is a dihydroxybenzene, a type of phenol, with two hydroxyl groups attached to a benzene ring. It functions as a reducing agent and is used in cosmetics to lighten skin by inhibiting melanin synthesis. It is also used in photographic development and as an antioxidant in various industrial processes.
Mequinol (4-methoxyphenol, C₇H₈O₂) is an aromatic compound and a hydroquinone derivative, where one of the hydroxyl groups of hydroquinone is replaced by a methoxy group (-OCH₃). This structure imparts similar properties to hydroquinone, allowing mequinol to function as a melanin synthesis inhibitor. It works by disrupting the enzyme tyrosinase, which is crucial in the melanin production pathway.
Hydroquinone, 4-Methoxyphenol can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 300 nm.
You can find detailed UV spectra of Hydroquinone, 4-Methoxyphenol and information about its various lambda maxima by visiting the following links: UV-Vis Spectrum of Hydroquinone, UV-Vis Spectrum of 4-methoxyphenol.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN – 10% |
| Buffer | H2SO4 -0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 300 nm |
| Limit of Detection | 1. 19.2 ppb, 2. 57.1 ppb |
| Class of Compounds | Phenols |
| Analyzing Compounds | Hydroquinone, 4-Methoxyphenol |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Hydroquinone
HPLC Separation of Acrylic Acid and 4- Methoxyphenol on Primesep B Column
September 9, 2019
Acrylic acid in combination with other monomers produces copolymers which are used in manufacturing of various products such as paints, adhesives and plastics. Esters and salts of acrylic acid are known as acrylates. 4-methoxyphenol, also known as mequinol, is an active ingredient in topical drugs and is also used for inhibition of radical polymerization of monomers like acrylates. The two compounds can be separated on a mixed-mode Primesep B column with isocratic method in mobile phase of 20/80 Acetonitrile (ACN) and water with 0.02% phosphoric acid buffer. UV Detection 200nm.
Condition
Column Primesep B, 4.6×150 mm, 5 µm, 100A Mobile Phase MeCN/H2O – 20/80% Buffer H3PO4 – 0.02 % Flow Rate 1.0 ml/min Detection UV, 200 nm
Description
Class of Compounds
Acid, Hydrophilic, Ionizable, Antioxidant, Inhibitor, Phenol, Carboxylic acid Analyzing Compounds Acrylic Acid, 4- Methoxyphenol, Mequinol
Application Column
Primesep B
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsAcrylic acid
Separation of 4-Methoxyphenol on Newcrom R1 HPLC column
February 16, 2018
4-Methoxyphenol can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options
