High Performance Liquid Chromatography (HPLC) Method for Analysis of Hydroquinone, 4-Methoxyphenol on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Hydroquinone, 4-Methoxyphenol
Hydroquinone (C₆H₄(OH)₂) is a dihydroxybenzene, a type of phenol, with two hydroxyl groups attached to a benzene ring. It functions as a reducing agent and is used in cosmetics to lighten skin by inhibiting melanin synthesis. It is also used in photographic development and as an antioxidant in various industrial processes.
Mequinol (4-methoxyphenol, C₇H₈O₂) is an aromatic compound and a hydroquinone derivative, where one of the hydroxyl groups of hydroquinone is replaced by a methoxy group (-OCH₃). This structure imparts similar properties to hydroquinone, allowing mequinol to function as a melanin synthesis inhibitor. It works by disrupting the enzyme tyrosinase, which is crucial in the melanin production pathway.
Hydroquinone, 4-Methoxyphenol can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 300 nm.
You can find detailed UV spectra of Hydroquinone, 4-Methoxyphenol and information about its various lambda maxima by visiting the following links: UV-Vis Spectrum of Hydroquinone, UV-Vis Spectrum of 4-methoxyphenol.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN – 10% |
| Buffer | H2SO4 -0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 300 nm |
| Limit of Detection | 1. 19.2 ppb, 2. 57.1 ppb |
| Class of Compounds | Phenols |
| Analyzing Compounds | Hydroquinone, 4-Methoxyphenol |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Hydroquinone
