m-Phenylenediamine

1,2-Phenylenediamine structural formula

CAS Number95-54-5
Molecular FormulaC6H8N2
Molecular Weight108.144
InChI KeyGEYOCULIXLDCMW-UHFFFAOYSA-N
LogP0.15
Synonyms
  • 1,2-Phenylenediamine
  • Benzene-1,2-diamine
  • 1,2-Benzenediamine
  • 95-54-5
  • o-Phenylenediamine
  • 1,2-Benzenediamine
  • 4-13-00-00038
  • 1,2-Diaminobenzene
  • 1,2-PHENYLENDIAMIN
  • 2-Aminoaniline
  • C.I. Oxidation Base 16
  • NSC 5354
  • o-Aminoaniline
  • o-Aminophenylamine
  • o-Benzenediamine
  • o-Diaminobenzene
  • o-fenilendiamina
  • o-Phenylendiamin
  • o-Phenylenediamine and its salts
  • Orthamine
  • PHENYLENE-1,2-DIAMINE
  • Phenylenediamine, ortho-
  • BRN 0606074
  • CI Oxidation Base 16
  • EINECS 202-430-6
  • 1,2-Fenylendiamin
  • o-Fenylendiamin
  • UNII-8B713N8Q0F
  • 2-Phenylene diamine
  • OPDA
  • phenylene-1,2-dimaine

Applications:

HPLC Method for Analysis of Isomers of Phenylenediamine on Primesep 100 Column

August 1, 2024

High Performance Liquid Chromatography (HPLC) Method for Analysis of m-Phenylenediamine, o-Phenylenediamine, p-Phenylenediamine on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of m-Phenylenediamine, o-Phenylenediamine, p-Phenylenediamine on Primesep 100 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of m-Phenylenediamine, o-Phenylenediamine, p-Phenylenediamine

o-Phenylenediamine, m-Phenylenediamine, and p-Phenylenediamine are isomers of phenylenediamine, where the amino groups are attached to different positions on the benzene ring.

These compounds are important in various industrial applications due to their role as intermediates in the synthesis of other chemicals.

m-Phenylenediamine, o-Phenylenediamine, p-Phenylenediamine can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 40%
BufferH2SO4 -0.1%
Flow Rate1.0 ml/min
DetectionUV 200 nm
Samples0.3 mg/ml in MeCN/H2O – 50/50%
Injection volume1 µl
LOD*10 ppb (200 nm)
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Aromatic amines
Analyzing Compoundsm-Phenylenediamine, o-Phenylenediamine, p-Phenylenediamine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
m-Phenylenediamine
o-Phenylenediamine
p-Phenylenediamine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of 1,2-Phenylenediamine on Newcrom R1 HPLC column

February 16, 2018
Separation of 1,2-Phenylenediamine on Newcrom C18 HPLC column

1,2-Phenylenediamine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
m-Phenylenediamine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.