| CAS Number | 95-54-5 |
|---|---|
| Molecular Formula | C6H8N2 |
| Molecular Weight | 108.144 |
| InChI Key | GEYOCULIXLDCMW-UHFFFAOYSA-N |
| LogP | 0.15 |
| Synonyms |
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Applications:
UV-Vis Spectrum of o-Phenylenediamine
October 3, 2024
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Analysis of Isomers of Phenylenediamine on Primesep 100 Column
August 1, 2024
High Performance Liquid Chromatography (HPLC) Method for Analysis of o-Phenylenediamine, , m-Phenylenediamine, p-Phenylenediamine on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of o-Phenylenediamine, , m-Phenylenediamine, p-Phenylenediamine
o-Phenylenediamine, m-Phenylenediamine, and p-Phenylenediamine are isomers of phenylenediamine, where the amino groups are attached to different positions on the benzene ring.
These compounds are important in various industrial applications due to their role as intermediates in the synthesis of other chemicals.
o-Phenylenediamine, , m-Phenylenediamine, p-Phenylenediamine can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN – 40% |
| Buffer | H2SO4 -0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Samples | 0.3 mg/ml in MeCN/H2O – 50/50% |
| Injection volume | 1 µl |
| LOD* | 10 ppb (200 nm) |
| Class of Compounds | Aromatic amines |
| Analyzing Compounds | o-Phenylenediamine, , m-Phenylenediamine, p-Phenylenediamine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
m-Phenylenediamine
o-Phenylenediamine
p-Phenylenediamine
Separation of 1,2-Phenylenediamine on Newcrom R1 HPLC column
February 16, 2018
1,2-Phenylenediamine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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