EDTA (Ethylenediaminetetraacetic Acid)

Separation type: Liquid Chromatography Mixed-mode

EDTA Standards Solution A:
For the preparation of the EDTA standard solution, 5 mg of EDTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in 0.001N NaOH water solution with sonication or magnetic stirrer mixing. Filtered The EDTA stock solution (1.0 mg/mL) should be stored in a cold dark place and can be used for a week to prepare standards of required concentration.

Iron(III) chloride Solution B:
The standard stock solution of Iron(III) chloride (10 mg/ml) was prepared in water. 50 mg of FeCl3 was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water, with sonication if needed.

General procedure for Ferric EDTA complex analysis:
To make a sample for analysis mix 100 µL Solution A (or unknown sample) with 100 µL Solution B and 800 µL of water.  Place this mixture in a plastic HPLC vial for analysis. Setup instrument and column according to the method provided.

High Performance Liquid Chromatography (HPLC) Method for Analysis of EDTA and Maleic Acid

New more reliable method for EDTA analysis is available

Separation type: Liquid Chromatography Mixed-mode

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Ethylenediaminetetraacetic acid (EDTA) is a very common chelating agent, used particularly for collecting Iron and Calcium ions. It has a wide range of applications, including in the textile industry, paper industry, pharmaceutical industry, and in cosmetics, where it is used to capture unwanted metal ions in solution. Maleic acid, a dicarboxylic acid, has a few interesting applications, such in medicine to form salts with drugs to increase their stability and as a precursor for the production of glyoxylic acid, which is used in cosmetics. An Iron (III)-EDTA complex and Maleic acid can both be retained, separated, and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of (mostly) water, Acetonitrile (MeCN), and Perchloric acid (HClO4). This analytical method can be UV detected at 220 nm with high resolution and peak symmetry.

High Performance Liquid Chromatography (HPLC) Method for Analysis of EDTA and  Maleic Acid

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NTA Standards Solution A: For the preparation of the NTA standard solution, 5 mg of NTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water with sonication. The NTA stock solution (1 mg/mL) should be stored in a cold, dark place and can be used for upto a week to prepare standards of required concentration.

Nitrilotriacetic acid (NTA) and Ethylenediaminetetraacetic acid (EDTA) are two common chelating agents used often in laboratory and medical testing. They are often used to bind to, identify, and analyze metal ions. Below is a method we developed to retain and measure Fe (III) using NTA and EDTA complexes on a Newcrom BH mixed-mode column, with a mobile phase of acetonitrile (MeCN), water, and a buffer of either sulfuric acid (H2SO4) or formic acid:

EDTA Standards Solution B: For the preparation of the EDTA standard solution, 5 mg of EDTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in 0.001N NaOH water solution with sonication or magnetic stirrer mixing. Filtered The EDTA stock solution (1.0 mg/mL)  should be stored in a cold dark  place and can be used for a week to prepare standards of required concentration.
NTA Standard – Solution A: For the preparation of the NTA standard solution, 5 mg of NTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water with sonication. The NTA stock solution (1 mg/mL) should be stored in a cold, dark place and can be used for upto a week to prepare standards of required concentration.

EDTA Standard – Solution B: For the preparation of the EDTA standard solution, 5 mg of EDTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in 0.001N NaOH water solution with sonication or magnetic stirrer mixing. Filter the EDTA stock solution (1.0 mg/mL) should be stored in a cold dark place and can be used for a week to prepare standards of required concentration.

Iron(III) chloride – Solution C: The standard stock solution of Iron(III) chloride (10 mg/ml) was prepared in water. 50 mg of FeCl3 was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water, with sonication if needed.

General procedure for Ferric NTA and EDTA complex analysis: To make a sample for analysis, mix 100 µL each Solution A and Solution B (or unknown sample) with 100 µL Solution C and 700 µL of water. Place this mixture in a plastic HPLC vial for analysis. Setup instrument and column according to the method provided.

Iron(III) chloride Solution C: The standard stock solution of Iron(III) chloride (10 mg/ml) was prepared in water. 50 mg of FeCl3 was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water, with sonication if needed.

General procedure for Ferric NTA and EDTA  complexes analysis: To make a sample for analysis mix 100 µL each Solution A and Solution B (or unknown sample) with 100 µL Solution C and 700 µL of water. Place this mixture in a plastic HPLC vial for analysis. Setup instrument and column according to the method provided.

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EDTA Standards Solution A:
For the preparation of the EDTA standard solution, 5 mg of EDTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in 0.001N NaOH water solution with sonication or magnetic stirrer mixing. Filtered The EDTA stock solution (1.0 mg/mL) should be stored in a cold dark place and can be used for a week to prepare standards of required concentration.

Iron(III) chloride Solution B:
The standard stock solution of Iron(III) chloride (10 mg/ml) was prepared in water. 50 mg of FeCl3 was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water, with sonication if needed.

General procedure for Ferric EDTA complex analysis:
To make a sample for analysis mix 100 µL Solution A (or unknown sample) with 100 µL Solution B and 800 µL of water.  Place this mixture in a plastic HPLC vial for analysis. Setup instrument and column according to the method provided.

New mo

Separation type: Liquid Chromatography Mixed-mode

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High Performance Liquid Chromatography (HPLC) Method for Analysis of EDTA, Oxymetazoline and Benzyl Alcohol

Benzyl alcohol is found in over-the-counter medications, topical creams, lotions, shampoos, and facial cleansers as an antibacterial, preservative, and/or fungicide. Benzyl alcohol is a colorless liquid with a mild pleasant aromatic scent. Benzyl alcohol is found in many essential oils including jasmine, hyacinth, neroli, rose, and ylang-ylang. Oxymetazoline is a decongestant that shrinks blood vessels in the nasal passages. All active compounds of nasal spray can be separated in HPLC on a Newcrom BH mixed-mode column. The analytical method uses acetonitrile (ACN) gradient and water with sulfuric acid (H2SO4) as buffer and UV detected at 260nm.
 

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EDTA Standards Solution A:
For the preparation of the EDTA standard solution, 5 mg of EDTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in 0.001N NaOH water solution with sonication or magnetic stirrer mixing. Filtered The EDTA stock solution (1.0 mg/mL) should be stored in a cold dark place and can be used for a week to prepare standards of required concentration.

Iron(III) chloride Solution B:
The standard stock solution of Iron(III) chloride (10 mg/ml) was prepared in water. 50 mg of FeCl3 was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water, with sonication if needed.

General procedure for Ferric EDTA complex analysis:
To make a sample for analysis mix 100 µL Solution A (or unknown sample) with 100 µL Solution B and 800 µL of water.  Place this mixture in a plastic HPLC vial for analysis. Setup instrument and column according to the method provided.

Ethylenediaminetetraacetic acid (EDTA) is a very common chelating agent, used particularly for collecting Iron and Calcium ions. It has a wide range of applications, including in the textile industry, paper industry, pharmaceutical industry, and in cosmetics, where it is used to capture unwanted metal ions in solution. An Iron (III)-EDTA complex can be retained and analyzed on a mixed-mode Newcrom B column with a mobile phase consisting of (mostly) water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 260 nm with high resolution and peak symmetry.

New more reliable method for EDTA analysis is available

EDTA, or Ethylenediaminetetraacetic acid, is widely used in textile, pulp/paper, food and pharmaceutical industries. It has application as chelating agent and preservative. EDTA molecule is very hydrophilic and contains two basic groups and four carboxylic groups. It has tendency to bind to metals, making analysis of EDTA by HPLC very challenging. EDTA is analyzed in the presence of copper sulfate as visualization agent on a Primesep SB column. The method can be used for EDTA determination in various mixtures and compositions using UV detector.

Citric acid, ascorbic acid, and EDTA are commonly used in food and pharmaceutical industry as preservatives. These compounds are very polar in nature. They are weak organic acids with limited UV activity. Retention and separation is achieved on HILIC mixed-mode Obelisc N column. All three compounds are retained by combination of strong HILIC and strong anion-exchange mechanisms. Separation can be monitored by ELSD, LC/MS, UV or Corona CAD. In contrast to other HILIC column, Obelisc N has two ionizable groups basic and acidic which provide ion-exchange interaction in addition to hydrophilic interaction. This allows to use less acetonitrile for HILIC separation.

New more reliable method for EDTA analysis is available
 

EDTA (Ethylenediaminetetraacetic acid) is amino acid based compound which is widely used in industrial cleaners, detergents, photography, agrochemicals, textile, and food industries. EDTA serves as preservative in packaged food, vitamins and pharmaceutical formulations. It is used in organic and analytical laboratories as scavenger of metals, buffer solution, complexometric titration, masking agent for metal determination, etc.
EDTA is very polar compound with strong chelating properties. Low UV activity and strong binding to metal ions makes HPLC analysis of EDTA very difficult. Reproducible method for determination of wide range on concentration of EDTA in various formulations is developed using Primesep D anion-exchange mixed mode column. Copper sulfate is used for visualization purposes to increase sensitivity of the method. EDTA forms UV active complex with some metal ions. This method can be used for highly accurate quantitation of EDTA in different mixtures and composition. Method demonstrates controllable retention and perfect peak shape. Equilibration of the column prior to analysis requires special attention.

If multiple injections produce different retention times, your column might not be equilibrated properly. Primesep D columns have a large ionic capacity towards anions. The shipping solvent for the Primesep D columns is ACN/water/TFA. The end point of gradient has only 4 mmol of sulfate ions. It takes over 5 hours to equilibrate a column with such low concentration. Before running your experiments you need to wash your column with 20% ACN and 0.2% of sulfuric acid for 1 hour. After you replace previous buffer/additive, you can set up your experiment. If you don’t change ionic modifier in your mobile phase the equilibration time is equivalent of  4-5 column volumes.

New more reliable method for EDTA analysis is available