Vancomycin hydrochloride

Vancomycin hydrochloride structural formula

CAS Number1404-93-9
Molecular FormulaC66H76Cl3N9O24
Molecular Weight1485.720
InChI KeyLCTORFDMHNKUSG-GJHKVECASA-N
LogP0.0173
Synonyms
  • Vancomycin hydrochloride
  • (1S,2R,18R,19R,22S,25R,28R,40R)-48-{[(2S,3R,4S,5S,6R)-3-{[(2S,4S,6S)-4-Amino-5-hydroxy-4,6-dimethyloxan-2-yl]oxy}-4,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}-22-(2-amino-2-oxoethyl)-5,15-dichloro-2,18,32,35,37-pentahydroxy-19-{[(2R)-4-methyl-2-(methylamino)pentanoyl]amino}-20,23,26,42,44-pentaoxo-7,13-dioxa-21,24,27,41,43-pentaazaoctacyclo[26.14.2.2~3,6~.2~14,17~.1~8,12~.1~29,33~.0~10,25~.0~34,39~]pentaconta-3,5,8(48),9,11,14,16,29(45),30,32,34,36,38,46,49-pentadecaene-40-carboxylic acid--hydrogen chloride (1/1) (non-preferred name)
  • 1404-93-9
  • Vancocin hydrochloride
  • Vancocine hydrochloride
  • Vancocin hydrochloride in plastic container
  • Vancomycin HCl
  • UNII-71WO621TJD
  • AeroVanc

Applications:

HPLC Determination of Vancomycin on Newcrom R1 Column

July 27, 2021

HPLC Method for Vancomycin hydrochloride on Newcrom R1 by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Vancomycin.

Vancomycin is a glycopeptide antibiotic with the chemical formula C66H75Cl2N9O24. It is used to treat severe but susceptible bacterial infections. Vancomycin is a branched tricyclic glycosylated nonribosomal peptide often reserved as the “drug of last resort”, used only after treatment with other antibiotics has failed. The WHO classifies it as a critically important human medicine, even including it on the List of Essential Medicines.

Vancomycin can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN), water and ammonium formate as a buffer. and detected by UV, ELSD, CAD or LC/MS.

Condition
Column Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase Gradient MeCN – 5-20%, 10 min
Buffer Ammonium Formate pH 3.0 – 10 mM
Flow Rate 1.0 ml/min
Detection 280 nm, CAD

Description
Class of Compounds Drug, Antibiotic
Analyzing Compounds Vancomycin hydrochloride

 

Application Column

Newcrom R1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Vancomycin hydrochloride
Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Vancomycin hydrochloride on Newcrom R1 HPLC column

February 16, 2018
Separation of Vancomycin hydrochloride on Newcrom C18 HPLC column

Vancomycin hydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Vancomycin hydrochloride
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.