CAS Number | 1404-93-9 |
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Molecular Formula | C66H76Cl3N9O24 |
Molecular Weight | 1485.720 |
InChI Key | LCTORFDMHNKUSG-GJHKVECASA-N |
LogP | 0.0173 |
Synonyms |
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Applications:
HPLC Determination of Vancomycin on Newcrom R1 Column
July 27, 2021
Separation type: Liquid Chromatography Reverse Phase.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Vancomycin.
Vancomycin is a glycopeptide antibiotic used to treat severe but susceptible bacterial infections. Vancomycin is a branched tricyclic glycosylated nonribosomal peptide often reserved as the “drug of last resort”, used only after treatment with other antibiotics has failed. Vancomycin is on a WHO list of essential medicines. Vancomycin can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN), water and ammonium formate as a buffer. and detected by UV, ELSD, CAD or LC/MS.
Column | Newcrom R1, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | Gradient MeCN – 5-20%, 10 min |
Buffer | Ammonium Formate pH 3.0 – 10 mM |
Flow Rate | 1.0 ml/min |
Detection | 280 nm, CAD |
Class of Compounds | Drug, Antibiotic |
Analyzing Compounds | Vancomycin |
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsSeparation of Vancomycin hydrochloride on Newcrom R1 HPLC column
February 16, 2018
Vancomycin hydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options