Separation type: Liquid Chromatography Reverse Phase.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Vancomycin.
Vancomycin is a glycopeptide antibiotic used to treat severe but susceptible bacterial infections. Vancomycin is a branched tricyclic glycosylated nonribosomal peptide often reserved as the “drug of last resort”, used only after treatment with other antibiotics has failed. Vancomycin is on a WHO list of essential medicines. Vancomycin can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN), water and ammonium formate as a buffer. and detected by UV, ELSD, CAD or LC/MS.
| Column | Newcrom R1, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 5-20%, 10 min |
| Buffer | Ammonium Formate pH 3.0 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | 280 nm, CAD |
| Class of Compounds | Drug, Antibiotic |
| Analyzing Compounds | Vancomycin |
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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