HPLC Determination of Vancomycin on Newcrom R1 Column

HPLC Method for Vancomycin hydrochloride on Newcrom R1 by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Vancomycin.

Vancomycin is a glycopeptide antibiotic with the chemical formula C66H75Cl2N9O24. It is used to treat severe but susceptible bacterial infections. Vancomycin is a branched tricyclic glycosylated nonribosomal peptide often reserved as the “drug of last resort”, used only after treatment with other antibiotics has failed. The WHO classifies it as a critically important human medicine, even including it on the List of Essential Medicines.

Vancomycin can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN), water and ammonium formate as a buffer. and detected by UV, ELSD, CAD or LC/MS.

Condition
Column Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase Gradient MeCN – 5-20%, 10 min
Buffer Ammonium Formate pH 3.0 – 10 mM
Flow Rate 1.0 ml/min
Detection 280 nm, CAD

Description
Class of Compounds Drug, Antibiotic
Analyzing Compounds Vancomycin hydrochloride

 

Application Column

Newcrom R1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Vancomycin hydrochloride
Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.