| CAS Number | 84-22-0 |
|---|---|
| Molecular Formula | C13H16N2 |
| Molecular Weight | 200.286 |
| InChI Key | BYJAVTDNIXVSPW-UHFFFAOYSA-N |
| LogP | 2.66 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Tetrahydrozoline Hydrochloride in Redness Relief Eye Drops on Newcrom R1 column
May 23, 2025
HPLC Method for Tetrahydrozoline on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Tetrahydrozoline
Tetrahydrozoline is a medication primarily used as a topical decongestant. It is commonly found in over-the-counter eye drops (e.g., Visine) and nasal sprays to relieve redness in the eyes or nasal congestion.
Sample Preparation Procedure for HPLC Analysis of Tetrahydrozoline Hydrochloride in Eye Drops:
- Dilution:
- Transfer 1.0 mL of the eye drops into a clean vial.
- Add 1.0 mL of HPLC-grade methanol to the vial (1:1 dilution).
- Close the vial tightly and shake manually for about 1 minute to ensure the solution is well mixed.
- Filtration:
- Draw the mixed solution into a clean syringe.
- Attach a 0.45 µm syringe filter and filter the solution into a clean HPLC vial.
- Sample Handling:
- Use the prepared sample directly for injection into the HPLC system.
- If not using immediately, store the vial at 4–8°C, protected from light.
Tetrahydrozoline can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, methanol (MeOH), and sulfuric acid. Detection is performed using UV at 275 nm.
| Column | Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeOH – 20-60%, 10 min |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 275 nm |
| LOD | 2.4 ppb |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
| Class of Compounds | Drug |
| Analyzing Compounds | Tetrahydrozoline |
Application Column
Newcrom R1
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Separation of Tetrahydrozoline on Newcrom R1 HPLC column
February 16, 2018
Tetrahydrozoline can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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