HPLC Method for Sulfur on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sulfur 

Sulfur is a non-metal with the symbol S and atomic number 16. Elemental sulfur is a yellow crystal at room temperature. It is one of the most abundant elements on Earth and in the universe. It is used primarily as a precursor to other chemicals or is converted to sulfuric acid.. You can find detailed UV spectra of Sulfur and information about its various lambda maxima by visiting the following link.

Sulfur can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.

HPLC Method for Sulfur on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sulfur

Sulfur is a non-metal with the symbol S and atomic number 16. Elemental sulfur is a yellow crystal at room temperature. It is one of the most abundant elements on Earth and in the universe. It is used primarily as a precursor to other chemicals or is converted to sulfuric acid.

Sample Preparation: Mix the oil sample with acetonitrile (MeCN) in a 1:20 ratio (oil : MeCN), cap the vial, and shake for 10 minutes or vortex thoroughly. After phase separation, carefully collect the MeCN layer, transfer it to an autosampler vial, and inject for sulfur analysis.

Sulfur can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of acetonitrile (MeCN). Detection is performed using UV.

Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Sulfur check our HPLC Applications library

UV/Vis.: λmax: 195, 222, 264, 280 nm

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The compound was dissolved in acetonitrile. This analysis was done with the mobile phase made of acetonitrile.

HPLC Method for Sulfur on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sulfur

Sulfur is a non-metal with the symbol S and atomic number 16. Elemental sulfur is a yellow crystal at room temperature. It is one of the most abundant elements on Earth and in the universe. It is used primarily as a precursor to other chemicals or is converted to sulfuric acid.

Sulfur can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of acetonitrile (MeCN). Detection is performed using UV.