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For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

HPLC Method for Shikimic acid on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Shikimic Acid

Shikimic Acid, also known as shikimate, is a well-known biochemical intermediary integral to the metabolic process found in plants and microorganisms. It was first isolated from the Japanese shikimi flower, the Japanese star anise, which is where it gets its name. It has the chemical formula C₇H₁₀O₅.
This cyclohexanecarboxylic acid can be detected in the low UV regime. Using a Newcrom BH mixed-mode column and a mobile phase consisting of (mostly) water and acetonitrile, shikimic acid can be retained, measured, and analyzed. This analysis method can be UV detected at 200 nm with high resolution.