HPLC Determination of Shikimic Acid on Newcrom BH Column

HPLC Method for Shikimic acid on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Shikimic Acid

Shikimic Acid, also known as shikimate, is a well-known biochemical intermediary integral to the metabolic process found in plants and microorganisms. It was first isolated from the Japanese shikimi flower, the Japanese star anise, which is where it gets its name. It has the chemical formula C7H10O5.
This cyclohexanecarboxylic acid can be detected in the low UV regime. Using a Newcrom BH mixed-mode column and a mobile phase consisting of (mostly) water and acetonitrile, shikimic acid can be retained, measured, and analyzed. This analysis method can be UV detected at 200 nm with high resolution.

Condition
Column Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 5/95%
Buffer H3PO4 – 0.02%
Flow Rate 1.0 ml/min
Detection UV 200 nm

Description
Class of Compounds Acid
Analyzing Compounds Shikimic acid

 

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Shikimic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.