HPLC Determination of Shikimic Acid on Newcrom BH Column

Separation type: Liquid Chromatography Mixed-mode

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High Performance Liquid Chromatography (HPLC) Method for Analysis of Shikimic Acid

Shikimic Acid is a well-known biochemical intermediary integral to the metabolic process found in plants and microorganisms. It was first isolated from the Japanese shikimi flower, which is where it gets its name.
This cyclohexanecarboxylic acid can be detected in the low UV regime. Using a Newcrom BH mixed-mode column and a mobile phase consisting of (mostly) water and acetonitrile, shikimic acid can be retained, measured, and analyzed. This analysis method can be UV detected at 200 nm with high resolution.


Column Newcrom BH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 5/95%
Buffer H3PO4 – 0.02%
Flow Rate 1.0 ml/min
Detection 200 nm


Class of Compounds Acid
Analyzing Compounds Shikimic Acid


SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Newcrom BH

The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 columns are standard reverse phase columns with octyldecyl silane chains (C18) attached to porous silica. We have also designed a line of Newcrom R1 columns with a new outer design. This column and corresponding adapter entirely eliminate the need for any high-pressure fittings or tubing as well as minimizing all possible dead volumes. Furthermore, if a leak ever occurs in the high-pressure column inlet, the mobile phase is contained within the column adapter (no external leakage).

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Application Analytes:
Shikimic acid