Separation type: Liquid Chromatography Mixed-mode
View on hplc.cloud
High Performance Liquid Chromatography (HPLC) Method for Analysis of Shikimic Acid
Shikimic Acid is a well-known biochemical intermediary integral to the metabolic process found in plants and microorganisms. It was first isolated from the Japanese shikimi flower, which is where it gets its name.
This cyclohexanecarboxylic acid can be detected in the low UV regime. Using a Newcrom BH mixed-mode column and a mobile phase consisting of (mostly) water and acetonitrile, shikimic acid can be retained, measured, and analyzed. This analysis method can be UV detected at 200 nm with high resolution.
Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 5/95% |
Buffer | H3PO4 – 0.02% |
Flow Rate | 1.0 ml/min |
Detection | 200 nm |
Class of Compounds | Acid |
Analyzing Compounds | Shikimic Acid |
The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 columns are standard reverse phase columns with octyldecyl silane chains (C18) attached to porous silica. We have also designed a line of Newcrom R1 columns with a new outer design. This column and corresponding adapter entirely eliminate the need for any high-pressure fittings or tubing as well as minimizing all possible dead volumes. Furthermore, if a leak ever occurs in the high-pressure column inlet, the mobile phase is contained within the column adapter (no external leakage).
Select options© SIELC Technologies. 2002 - 2023
Address: 804 Seton Court, Wheeling, IL USA 60090
Tel: (847) 229-2629 | Fax: (847) 655-6079
Sales, Refund and Returns Policy
Email: mail@sielc.com | Sitemap