HPLC Determination of Shikimic Acid on Newcrom BH Column

Separation type: Liquid Chromatography Mixed-mode

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High Performance Liquid Chromatography (HPLC) Method for Analysis of Shikimic Acid
Shikimic Acid is a well-known biochemical intermediary integral to the metabolic process found in plants and microorganisms. It was first isolated from the Japanese shikimi flower, which is where it gets its name. This cyclohexanecarboxylic acid can be detected in the low UV regime. Using a Newcrom BH mixed-mode column and a mobile phase consisting of (mostly) water and acetonitrile, shikimic acid can be retained, measured, and analyzed. This analysis method can be UV detected at 200 nm with high resolution.

Condition

Column Newcrom BH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 5/95%
Buffer H3PO4 - 0.02%
Flow Rate 1.0 ml/min
Detection 200 nm

Description

Class of Compounds Acid
Analyzing Compounds Shikimic Acid
 
Application Analytes:
Shikimic acid