Seleno-L-cystine

CAS Number29621-88-3
Molecular FormulaC6H12N2O4Se2
Molecular Weight334.1
InChI KeyJULROCUWKLNBSN-IMJSIDKUSA-N
Synonyms
  • L-Selenocystine
  • Seleno-L-cystine
  • 29621-88-3
  • L-3,3'-Diselenodialanine
  • C6H12N2O4Se2
  • Alanine, 3,3'-diselenodi-, L-
  • L-Alanine,3,3'-diselenobis-
  • UNII-261B157KR8
  • 261B157KR8
  • L-Selenocystine, 98%
  • Selenocystine, L-
  • 1464-43-3
  • Seleno-L-cystine, 95%
  • selenocystine, (L)-isomer
  • SCHEMBL160609
  • CTK4G3564
  • AKOS015915762
  • LS-16101
  • S0900
  • X7627
  • C05704
  • (R,R)-3,3'-Diselenobis(2-aminopropionic Acid)
  • J-017582
  • Q27254027
  • UNII-493154PTZ7 component JULROCUWKLNBSN-IMJSIDKUSA-N
  • UNII-N27T3B3MVN component JULROCUWKLNBSN-IMJSIDKUSA-N
  • (2R)-2-amino-3-[[(2R)-2-amino-2-carboxy-ethyl]diselanyl]propanoic acid

Applications:

HPLC Determination of Seleno Amino Acids on Newcrom AH Column

September 22, 2020

HPLC Method for Seleno-L-cystine, Selenomethionine, Methylselenocysteine on Newcrom AH by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Seleno-L-cystine, Selenomethionine, Methylselenocysteine.

Methylselenocysteine is used in chemotherapy to induce apoptosis (cell death) of the cancerous cells.  Methylselenocysteine is an amino acid with antioxidant properties and a main source of selenium in several foods.  Selenocysteine is an analog of cysteine with the selenium replacing sulfur.  It has been used as a radioactive tracer.  All three analytes are zwitterions and have similar structures which can present difficulties in reversed-phase separation in HPLC.  By using a mixed mode Newcrom AH column which has ion-exchange properties in addition to hydrophobic properties allows the separation of the seleno amino acids with an MS-compatible mobile phase of water and acetonitrile (ACN) with formic acid buffer.

Condition
Column Newcrom AH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 20%
Buffer Formic Acid – 4%
Flow Rate 1.0 ml/min
Detection CAD

 

Description
Class of Compounds
 Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Seleno-L-cystine, Selenomethionine, Methylselenocysteine

 

Application Column

Newcrom AH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Methylselenocysteine
Seleno-L-cystine
Selenomethionine
Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.