Methylselenocysteine is used in chemotherapy to induce apoptosis (cell death) of the cancerous cells. Methylselenocysteine is an amino acid with antioxidant properties and a main source of selenium in several foods. Selenocysteine is an analog of cysteine with the selenium replacing sulfur. It has been used as a radioactive tracer. All three analytes are zwitterions and have similar structures which can present difficulties in reversed-phase separation in HPLC. By using a mixed mode Newcrom AH column which has ion-exchange properties in addition to hydrophobic properties allows the separation of the seleno amino acids with an MS-compatible mobile phase of water and acetonitrile (ACN) with formic acid buffer.
| Column | Newcrom AH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 20% |
| Buffer | Formic Acid – 4% |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
| Analyzing Compounds | Methylselenocysteine, Selenomethionine, Seleno-L-cystine |
Application Column
Newcrom AH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsSeleno-L-cystine
Selenomethionine
