HPLC Determination of Seleno Amino Acids on Newcrom AH Column

HPLC.cloud View on hplc.cloud Methylselenocysteine is used in chemotherapy to induce apoptosis (cell death) of the cancerous cells.  Methylselenocysteine is an amino acid with antioxidant properties and a main source of selenium in several foods.  Selenocysteine is an analog of cysteine with the selenium replacing sulfur.  It has been used as a radioactive tracer.  All three analytes are zwitterions and have similar structures which can present difficulties in reversed-phase separation in HPLC.  By using a mixed mode Newcrom AH column which has ion-exchange properties in addition to hydrophobic properties allows the separation of the seleno amino acids with an MS-compatible mobile phase of water and acetonitrile (ACN) with formic acid buffer.


Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 20%
Buffer Formic Acid - 4%
Flow Rate 1.0 ml/min
Detection CAD


Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Methylselenocysteine, Selenomethionine, Seleno-L-cystine
Application Analytes: