Ractopamine hydrochloride

Ractopamine hydrochloride structural formula

 

CAS Number90274-24-1
Molecular FormulaC18H24ClNO3
Molecular Weight337.840
InChI KeyJHGSLSLUFMZUMK-UHFFFAOYSA-N
LogP1.92
Synonyms
  • Ractopamine hydrochloride
  • 4-(1-Hydroxy-2-{[4-(4-hydroxyphenyl)butan-2-yl]amino}ethyl)phenol--hydrogen chloride (1/1)
  • Benzenemethanol, 4-hydroxy-alpha-[[[3-(4-hydroxyphenyl)-1-methylpropyl]amino]methyl]-, hydrochloride (1:1)
  • 90274-24-1
  • Benzenemethanol, 4-hydroxy-alpha-(((3-(4-hydroxyphenyl)-1-methylpropyl)amino)methyl)-, hydrochloride
  • Ractopamine HCl
  • UNII-309G9J93TP
  • Optaflexx 45

Applications:

UV-Vis Spectrum of Ractopamine

June 28, 2024

 

UV-Vis Spectrum of Ractopamine

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.

Application Analytes:
Ractopamine hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of Ractopamine on Primesep 100 Column

July 26, 2019

Ractopamine is a livestock feed additive designed to promote muscle synthesis and leanness. It is banned in many countries, excluding the US, Canada and Mexico. It can be retained on a Primesep 100 Column in about 7 minutes. Primesep 100 is a reverse-phase (RP) mixed-mode column with embedded acidic ion-pairing groups.

 

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer H2SO4 – 0.3 %
Flow Rate 1.0 ml/min
Detection UV, 222 nm

 

Description

Class of Compounds
Drug, Basic, Hydrophobic, Ionizable, Amine
Analyzing Compounds Ractopamine

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Ractopamine hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Ractopamine hydrochloride on Newcrom R1 HPLC column

February 16, 2018
Separation of Ractopamine hydrochloride on Newcrom C18 HPLC column

Ractopamine hydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Ractopamine hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.