| CAS Number | 90274-24-1 |
|---|---|
| Molecular Formula | C18H24ClNO3 |
| Molecular Weight | 337.840 |
| InChI Key | JHGSLSLUFMZUMK-UHFFFAOYSA-N |
| LogP | 1.92 |
| Synonyms |
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Applications:
UV-Vis Spectrum of Ractopamine
June 28, 2024
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
HPLC Determination of Ractopamine on Primesep 100 Column
July 26, 2019
Ractopamine is a livestock feed additive designed to promote muscle synthesis and leanness. It is banned in many countries, excluding the US, Canada and Mexico. It can be retained on a Primesep 100 Column in about 7 minutes. Primesep 100 is a reverse-phase (RP) mixed-mode column with embedded acidic ion-pairing groups.
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H2SO4 – 0.3 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 222 nm |
| Class of Compounds |
Drug, Basic, Hydrophobic, Ionizable, Amine |
| Analyzing Compounds | Ractopamine |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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Separation of Ractopamine hydrochloride on Newcrom R1 HPLC column
February 16, 2018
Ractopamine hydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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