HPLC Method for Ractopamine hydrochloride on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ractopamine hydrochloride.
Ractopamine is a livestock feed additive designed to promote muscle synthesis and leanness. It is banned in many countries, excluding the US, Canada and Mexico. It can be retained on a Primesep 100 Column in about 7 minutes. Primesep 100 is a reverse-phase (RP) mixed-mode column with embedded acidic ion-pairing groups.
| Column | Primesep 100 |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H2SO4 – 0.3 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 222 nm |
| Class of Compounds |
Drug, Basic, Hydrophobic, Ionizable, Amine |
| Analyzing Compounds | Ractopamine hydrochloride |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
Ractopamine hydrochlorideApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
