Oxytocin

Oxytocin structural formula

CAS Number50-56-6
Molecular FormulaC43H66N12O12S2
Molecular Weight1007.191
InChI KeyXNOPRXBHLZRZKH-DSZYJQQASA-N
LogP-2.48
Synonyms
  • Oxytocin
  • 1-{(4R,7S,10S,13S,16S,19R)-19-Amino-7-(2-amino-2-oxoethyl)-10-(3-amino-3-oxopropyl)-13-[(2S)-butan-2-yl]-16-[(4-hydroxyphenyl)methyl]-6,9,12,15,18-pentaoxo-1,2-dithia-5,8,11,14,17-pentaazacycloicosane-4-carbonyl}-L-prolyl-L-leucylglycinamide
  • Glycinamide, 1-[[(4R,7S,10S,13S,16S,19R)-19-amino-7-(2-amino-2-oxoethyl)-10-(3-amino-3-oxopropyl)-16-[(4-hydroxyphenyl)methyl]-13-[(1S)-1-methylpropyl]-6,9,12,15,18-pentaoxo-1,2-dithia-5,8,11,14,17-pentaazacycloeicos-4-yl]carbonyl]-L-prolyl-L-leucyl-
  • 50-56-6
  • [1-Hemicystine]-oxytocin
  • 3-Isoleucine-8-leucine vasopressin
  • Alpha-hypophamine
  • Atonin O
  • Atonin O, 3-L-isoleucine-8-L-leucine-
  • Di-sipidin
  • Endopituitrina
  • Glycinamide, L-cysteinyl-L-tyrosyl-L-isoleucyl-L-glutaminyl-L-asparaginyl-L-cysteinyl-L-prolyl-L-leucyl-, cyclic (1?6)-disulfide
  • Hyphotocin
  • Intertocine S
  • L-Cysteinyl-L-tyrosyl-L-isoleucyl-L-glutaminyl-L-asparaginyl-L-cysteinyl-L-prolyl-L-leucylglycinamide cyclic (1?6)-disulfide
  • Nobitocin S
  • Orasthin
  • oxitocina (Spanish) (EINECS)
  • Oxystin
  • Oxytocin (English, German) (DSL, EINECS, ENCS, AICS, ASIA-PAC, NZIoC)
  • Oxytocine (French) (DSL, EINECS)
  • Partocon
  • Perlacton
  • Pitocin
  • Piton S
  • Presoxin
  • Synpitan forte
  • Synpitan
  • Synthetic oxytocin
  • Syntocin
  • Syntocinon
  • Syntocinone
  • Uteracon
  • Vasopressin, 3-L-isoleucine-8-L-leucine-
  • ?-Hypophamine
  • BRN 3586108
  • L-Cysteinyl-L-tyrosyl-L-isoleucyl-L-glutaminyl-L-asparaginyl-L-cysteinyl-L-prolyl-L-leucylglycinamide cyclic (1-6)-disulfide
  • EINECS 200-048-4
  • (1-Hemicystine)oxytocin
  • Ocytocinum
  • Posterior pituitary extract
  • Utedrin
  • Ossitocina
  • Oxetakain
  • Oxitocina
  • Oxytocine
  • Oxytocinum
  • UNII-1JQS135EYN
  • Oxoject
  • (2S)-2-[(1-{[(4R,7S,10S,13S,16S,19R)-19-amino-13-[(2S)-butan-2-yl]-10-(2-carbamoylethyl)-7-(carbamoylmethyl)-16-[(4-hydroxyphenyl)methyl]-6,9,12,15,18-pentaoxo-1,2-dithia-5,8,11,14,17-pentaazacycloicosan-4-yl]carbonyl}pyrrolidin-2-yl)formamido]-N-(carbamoylmethyl)-4-methylpentanamide(Arg8)-Vasopressin
  • (Arg8)-Vasotocin
  • Argipressin
  • Argpressin Acetate
  • Biotinyl-(Arg8)-Vasopressin
  • Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Arg-Gly-NH2
  • Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Leu-Gly-NH2
  • Cys-Tyr-Ile-Thr-Asn-Cys-Gly-Leu-Gly-NH2
  • Cys-Tyr-Phe-Gln-Asn-Cys-Pro-Arg-Gly-NH2
  • Cys-Tyr-Phe-Gln-Asn-Cys-Pro-Lys-Gly-NH2
  • Disulfide bridge Cys1-Cys6
  • Gly-Leu-Pro-c
  • L-Cysteinyl-L-tyrosyl-L-isoleucyl-L-glutaminyl-L-asparaginyl-L-cysteinyl-L-prolyl-L-leucylglycinamide cyclic(1-6)-disulfide
  • OT
  • OXT
  • Ocytocin
  • Oxytocin 10 Usp Units In Dextrose 5%
  • Oxytocin 20 Usp Units In Dextrose 5%
  • Oxytocin 5 Usp Units In Dextrose 5%
  • Oxytocin Acetate
  • Oxytocin injection

Applications:

HPLC-UV Method for Analysis of Oxytocin on Primesep 200 Column

September 6, 2023

High Performance Liquid Chromatography (HPLC) Method for Analysis of Oxytocin on Primesep 200 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC-UV Method for Analysis of Oxytocin on Primesep 200 Column  by SIELC Technologies
HPLC Method for Analysis of Oxytocin on Primesep 200 Column by SIELC Technologies

Oxytocin is a neuropeptide and a hormone that plays a significant role in social bonding, sexual reproduction, childbirth, and the period after childbirth.

Oxytocin is a peptide hormone and neuropeptide that plays a crucial role in social bonding, reproduction, and childbirth. It is often called the “love hormone” or “bonding hormone” because of its association with social interactions, including maternal behaviors, bonding between couples, and even the bond between humans and their pets.

Oxytocin is a neuropeptide and a hormone that plays a significant role in social bonding, sexual reproduction, childbirth, and the period after childbirth. Here’s a breakdown of its classification and functions:

1. Chemical Classification:

  • Oxytocin is a peptide hormone, meaning it’s made up of a short chain of amino acids. Specifically, oxytocin is a nonapeptide (consists of nine amino acids).
  • Its chemical structure is Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Leu-Gly, and it has a disulfide bond between the two cysteine residues.

2. Production and Release:

  • Oxytocin is produced in the hypothalamus and stored in and secreted by the posterior pituitary gland.
  • It’s released into the bloodstream in response to various stimuli, such as stretching of the cervix and uterus during labor, nipple stimulation from breastfeeding, and more.

3. Functions:

  • Reproductive System: Oxytocin facilitates uterine contractions during childbirth and helps the milk ejection reflex during breastfeeding.
  • Social Behavior: There’s evidence suggesting that oxytocin plays a role in social bonding, attachment, and even trust between individuals. It’s sometimes referred to as the “love hormone” or “bonding hormone” because of these effects.
  • Stress and Anxiety: Oxytocin may have a protective role in stress situations, potentially reducing anxiety and encouraging social interaction.

Oxytocin can be retained, and analyzed on a Primesep 200 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid as a buffer. This analysis method can be detected in the UV 200 nm.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Oxytocin

Condition

ColumnPrimesep 200, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN – 10-70%, 10 min
BufferH2SO4 – 0.2%
Flow Rate0.2 ml/min
DetectionUV 200 nm
Peak Retention Time7.77 min

Description

Class of CompoundsNeuropeptide, Hormone
Analyzing CompoundsOxytocin

Application Column

Primesep 200

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Oxytocin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC-MS Method for Analysis of Oxytocin on Primesep 200 Column

September 1, 2023

High Performance Liquid Chromatography (HPLC) Method for Analysis of Oxytocin on Primesep 200 by SIELC Technologies.

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Oxytocin on Primesep 200 Column by SIELC Technologies

Oxytocin is a neuropeptide and a hormone that plays a significant role in social bonding, sexual reproduction, childbirth, and the period after childbirth.

Oxytocin is a peptide hormone and neuropeptide that plays a crucial role in social bonding, reproduction, and childbirth. It is often called the “love hormone” or “bonding hormone” because of its association with social interactions, including maternal behaviors, bonding between couples, and even the bond between humans and their pets.

Oxytocin is a neuropeptide and a hormone that plays a significant role in social bonding, sexual reproduction, childbirth, and the period after childbirth. Here’s a breakdown of its classification and functions:

1. Chemical Classification:

  • Oxytocin is a peptide hormone, meaning it’s made up of a short chain of amino acids. Specifically, oxytocin is a nonapeptide (consists of nine amino acids).
  • Its chemical structure is Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Leu-Gly, and it has a disulfide bond between the two cysteine residues.

2. Production and Release:

  • Oxytocin is produced in the hypothalamus and stored in and secreted by the posterior pituitary gland.
  • It’s released into the bloodstream in response to various stimuli, such as stretching of the cervix and uterus during labor, nipple stimulation from breastfeeding, and more.

3. Functions:

  • Reproductive System: Oxytocin facilitates uterine contractions during childbirth and helps the milk ejection reflex during breastfeeding.
  • Social Behavior: There’s evidence suggesting that oxytocin plays a role in social bonding, attachment, and even trust between individuals. It’s sometimes referred to as the “love hormone” or “bonding hormone” because of these effects.
  • Stress and Anxiety: Oxytocin may have a protective role in stress situations, potentially reducing anxiety and encouraging social interaction.

Oxytocin can be retained, and analyzed on a Primesep 200 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Ammonium format as a buffer. This analysis method can be detected in the UV 275 nm.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Oxytocin

Condition

ColumnPrimesep 200, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 30/70%
BufferAmmonium Format pH 3.0- 20 mM
Flow Rate0.2 ml/min
DetectionUV 275 nm, LCMS SIM 1008+
Peak Retention Time4.77 min

Description

Class of CompoundsNeuropeptide, Hormone
Analyzing CompoundsOxytocin

Application Column

Primesep 200

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Oxytocin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Oxytocin on Newcrom R1 HPLC column

May 16, 2018
Separation of Oxytocin on Newcrom R1 HPLC column

Oxytocin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Oxytocin
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.