N-(4-Hydroxy-3-nitrophenyl)acetamide

N-(4-Hydroxy-3-nitrophenyl)acetamide structural formula

CAS Number51288-37-0
Molecular FormulaC8H8N2O4
Molecular Weight196.163
InChI KeyXZYRROIQJHLVRG-UHFFFAOYSA-N
LogP1.23
Synonyms
  • N-(4-Hydroxy-3-nitrophenyl)acetamide
  • Acetamide, N-(4-hydroxy-3-nitrophenyl)-
  • 51288-37-0
  • EINECS 257-113-5
  • NSC 212489

Applications:

Separation of N-(4-Hydroxy-3-nitrophenyl)acetamide on Newcrom R1 HPLC column

February 16, 2018
Separation of N-(4-Hydroxy-3-nitrophenyl)acetamide on Newcrom C18 HPLC column

N-(4-Hydroxy-3-nitrophenyl)acetamide can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
N-(4-Hydroxy-3-nitrophenyl)acetamide
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.