| CAS Number | 22071-15-4 |
|---|---|
| Molecular Formula | C16H14O3 |
| Molecular Weight | 254.285 |
| InChI Key | DKYWVDODHFEZIM-UHFFFAOYSA-N |
| LogP | 3.1 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Ketoprofen
March 12, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Ketoprofen check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
USP Methods for the Analysis Ketoprofen using the Legacy L1 Column
February 20, 2019
HPLC Method for Ketoprofen on Legacy L1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ketoprofen.
Ketoprofen is a prescription anti-inflammatory drug (NSAID) that is used to treat pain, inflammation, and fever. It is also used in veterinary medicine. It works through inhibiting cyclooxygenase enzymes, which prevents the synthesis of prostaglandins. It can be administered through capsules, topical gel, injections, and patches. It has the chemical formula C16H14O3.
Ketoprofen can be retained and analyzed using the Legacy L1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
| Column | Legacy L1, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 43/55% |
| Buffer | NaHPO4 pH3.5 – 2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 233 nm |
| Class of Compounds |
Drug, Acid, Ionizable, Hydrophobic |
| Analyzing Compounds | Ketoprofen |
Application Column
Legacy L1
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Method for Analysis of Ketoprofen
May 3, 2016
Ketoprofen is a nonsteroidal anti-inflammatory drug (NSAID) and an Ibuprofen-type anti-inflammatory analgesic and antipyretic that is used to treat rheumatoid arthritis and osteoarthritis. It can lead to a higher chance of a stroke. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Ketoprofen. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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