Indigo

Indigo structural formula

CAS Number482-89-3
Molecular FormulaC16H10N2O2
Molecular Weight262.268
InChI KeyCOHYTHOBJLSHDF-BUHFOSPRSA-N
LogP3.72
Synonyms
  • Indigo
  • (2E)-2-(3-Oxo-1,3-dihydro-2H-indol-2-ylidene)-1,2-dihydro-3H-indol-3-one
  • 3H-Indol-3-one, 2-(1,3-dihydro-3-oxo-2H-indol-2-ylidene)-1,2-dihydro-, (2E)-
  • 482-89-3
  • C.I Vat Blue 1
  • C.I. 73000
  • 2-(1,3-Dihydro-3-oxo-2H-indol-2-ylidene)-1,2- dihydro-3H-indol-3-one
  • 2-(3-Oxo-1,3-dihydro-2H-indol-2-ylidene)-1,2-dihydro-3H-indol-3-one
  • 11669 Blue
  • 2,2'-Bis(2,3-dihydro-3-oxoindolylidene)
  • Bezema Indigo AXX-FL
  • C.I. Natural Blue 1
  • D+C Blue No. 6
  • Dystar Indigo Gr
  • Indigo Powder W
  • Indigotin (natural)
  • Japan Blue 201
  • Natural Blue 1
  • Reduced Dark Blue VB
  • Vat Dark Blue VB
  • Indigotine
  • C Blue 22
  • Vat Blue 1
  • 3H-Indol-3-one, 2-(1,3-dihydro-3-oxo-2H-indol-2-ylidene)-1,2-dihydro-(Indigo Blue)
  • 2-(1,3-dihydro-3-oxo-2H-indol-2-ylidene)-1,2-dihydro-3H-indol-3-one
  • 5-24-08-00503
  • 3H-Indol-3-one, 2-(1,3-dihydro-3-oxo-2H-indol-2-ylidene)-1,2-dihydro-
  • [?2,2'(3H,3'H)-Biindole]-3,3'-dione
  • [?2,2'-Biindoline]-3,3'-dione
  • 2-(1,3-dihidro-3-oxo-2H-indazol-2-ilideno)-1,2-dihidro-3H-indol-3-ona
  • 2-(1,3-Dihydro-3-oxo-2H-indazol-2-yliden)-1,2-dihydro-3H-indol-3-on
  • 2-(1,3-Dihydro-3-oxo-2H-indazol-2-ylidene)-1,2-dihydro-3H-indol-3-one
  • 2-(1,3-dihydro-3-oxo-2H-indazole-2-ylidene)-1,2-dihydro-3H-indole-3-one
  • C.I. PIGMENT BLUE 66
  • C.I. vat blue 001
  • C.I. VAT BLUE 1
  • Cystoceva
  • Diindogen
  • INDIGO BLUE
  • Indigo Ciba
  • Indigo Ciba SL
  • Indigo J
  • Indigo N
  • Indigo NAC
  • Indigo NACCO
  • Indigo P
  • Indigo PLN
  • Indigo Pure BASF
  • Indigo Synthetic
  • Indigo VS
  • Mitsui Indigo Paste
  • Mitsui Indigo Pure
  • Mitsui Indigo Pure EXN
  • Natural blue indigotin
  • Pigment Indigo
  • Pigment Indigo V
  • Synthetic Indigo
  • Synthetic Indigo TS
  • ?2,2'-Bipseudoindoxyl
  • D & C Blue No. 6
  • D and C Blue No. 6
  • D&C Blue No. 6
  • (delta2,2'(3H,3'H)-Biindole)-3,3'-dione
  • (2,2'-Biindoline)-3,3'-dione
  • delta2,2'-Bipseudoindoxyl
  • Blue No. 201
  • BRN 0088275
  • CI Pigment Blue 66
  • CI Vat Blue 1
  • EINECS 207-586-9
  • Indigo Pure BASF Powder K
  • Indigotin
  • Lithosol Deep Blue B
  • Lithosol Deep Blue V
  • Monolite Fast Navy Blue BV
  • NCI-C61392
  • NSC 8645
  • Pigment Blue 66
  • delta(sup 2,2')-Bipseudoindoxyl
  • Vulcafix Blue R
  • Vulcafor Blue A
  • Vulcanosine Dark Blue L
  • Vulcol Fast Blue GL
  • Vynamon Blue A
  • Modr Kypova 1
  • UNII-1G5BK41P4F
  • 11129-41-2
  • 1131642-59-5
  • 12000-74-7
  • 12626-73-2
  • 136797-30-3
  • 210488-46-3
  • 908005-94-7
  • 93660-98-1
  • 2089217-02-5
  • 2089217-38-7

Applications:

Separation of Indigo on Newcrom R1 HPLC column

February 16, 2018
Separation of Indigo on Newcrom C18 HPLC column

Indigo can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Indigo
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.