| CAS Number | 118-42-3 |
|---|---|
| Molecular Formula | C18H26ClN3O |
| Molecular Weight | 335.881 |
| InChI Key | XXSMGPRMXLTPCZ-UHFFFAOYSA-N |
| LogP | 3.59 |
| Synonyms |
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Applications:
HPLC Determination of Hydroxychloroquine on Newcrom A Column
March 26, 2020
HPLC Method for Hydroxychloroquine on Newcrom A by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Hydroxychloroquine.
Hydroxychloroquine is a prescription medication with the chemical formula C18H26ClN3O. It is used to treat and prevent acute attacks of malaria in adults and children. It is not used in severe or complicated cases. Besides malaria, Hydroxychloroquine is also used to treat discoid lupus erythematosus (DLE), systemic lupus erythematosus (SLE), and chronic rheumatoid arthritis. It is considered unsafe to take this medication alongside other medications.
Hydroxychloroquine can be retained and analyzed using the Newcrom A stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Ammonium Formate buffer. Detection is performed using UV.
| Column | Newcrom A, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | AmFm pH 3.0- 40 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 256 nm, MS-compatible mobile phase |
| Class of Compounds | Hydrophobic, Drug |
| Analyzing Compounds | Hydroxychloroquine |
Application Column
Newcrom A
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Determination of Hydroxychloroquine on Obelisc R Column
March 26, 2020
HPLC Method for Hydroxychloroquine on Obelisc R by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Hydroxychloroquine.
Hydroxychloroquine is a prescription medication with the chemical formula C18H26ClN3O. It is used to treat and prevent acute attacks of malaria in adults and children. It is not used in severe or complicated cases. Besides malaria, Hydroxychloroquine is also used to treat discoid lupus erythematosus (DLE), systemic lupus erythematosus (SLE), and chronic rheumatoid arthritis. It is considered unsafe to take this medication alongside other medications.
Hydroxychloroquine can be retained and analyzed using the Obelisc R stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.
| Column | Obelisc R, 4.6 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | AmFm pH 3.0- 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 270 nm, MS- compatible mobile phase |
| Class of Compounds | Hydrophobic, Drug |
| Analyzing Compounds | Hydroxychloroquine |
Application Column
Obelisc R
Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Separation of Hydroxychloroquine on Newcrom R1 HPLC column
May 16, 2018
Hydroxychloroquine can be analyzed by this reverse phase (RP) HPLC method with simple conditions.
The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid.
For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications.
This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options
