Column | Obelisc R, 4.6×100 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 50/50% |
Buffer | AmFm pH 3.0- 30 mM |
Flow Rate | 1.0 ml/min |
Detection | UV 270 nm, MS- compatible mobile phase |
Class of Compounds | Hydrophobic, Drug |
Analyzing Compounds | Hydroxychloroquine |
Application Column
Obelisc R
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
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