HPLC Separation of Growth Regulators (Paclobutrazol, Uniconazole, Daminozide) on Newcrom A Column

HPLC Method for Paclobutrazol, Uniconazole-P, Daminozide on Newcrom A by SIELC Technologies

 

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Paclobutrazol, Uniconazole-P, Daminozide.

Daminozide is a plant growth regulator with the chemical formula C6H12N2O3. It has a variety of alternate names including: aminozide, Alar, Kylar, SADH, B-995, B-nine, and DMASA. As a pesticide, works through inhibiting the production of gibberellins. It was typically used in trees that produced fruit for consumption, but was withdrawn due to concerns of it being a carcinogen. It is still occasionally used in ornamental plants.

Paclobutrazol (PBZ) is an organic fungicide with the chemical formula C15H20ClN3O. It works through inhibiting the production of gibberellins, which are plant growth hormones. Through reducing reducing internodal growth, it causes stronger stems, greater root growth, and earlier fruitset. It can also reduce frost sensitivity in plants.

Uniconazole is a plant growth regulator with the chemical formula C15H18ClN3O. As a pesticide, works through inhibiting the production of gibberellins, which are plant growth hormones. It is typically used in perennials and ornamental plants to ensure they are a marketable size before they bloom.

Paclobutrazol, Uniconazole-P, Daminozide can be retained and analyzed using the Newcrom A stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Formic Acid buffer. Detection is performed using CAD.

Condition 1

Column Newcrom A, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O
Buffer Formic Acid
Flow Rate 1.0 ml/min
Detection CAD, UV 268 nm

 

Condition 2

Column Newcrom A, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV 210 nm

Description

Class of Compounds Hydrophobic, Herbicide, Pesticide
Analyzing Compounds Paclobutrazol, Uniconazole-P, Daminozide

Application Column

Newcrom A

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Daminozide
Paclobutrazol
Uniconazole-P

Application Detection:
UV Detection
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.