HPLC Method for Glucose 6-Phosphate, Glucose 1- Phosphate on Newcrom B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Glucose 6-Phosphate, Glucose 1- Phosphate.
Structural isomer separation poses a challenging task when using reverse-phase chromatography. However both glucose
variants, which only differ to which carbon the phosphate group is attached to, can be separated in HPLC isocratically on a
reverse-phase mixed-mode anion-exchange Newcrom B column.
What differentiates, the Newcrom B phase from other silica-based
Analysis of glucose phosphate is important because glucose-6-phosphate is the product of the first step in glycolysis
metabolic pathway and glucose-1-phosphate is the first step in glycogenolysis. Glucose-6-phosphate is converted to
glucose-1-phosphate and vice versa by the phosphoglucomutase enzyme in biological systems.
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, 40 °C MS- compatible mobile phase |
| Class of Compounds | Hydrophilic, Sugar |
| Analyzing Compounds | Glucose 6-Phosphate, Glucose 1- Phosphate |
Application Column
Newcrom B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Glucose 6-Phosphate
