Glucose 1- Phosphate

CAS Number59-56-3
Molecular FormulaC6H13O9P
Molecular Weight230.14
InChI KeyHXXFSFRBOHSIMQ-VFUOTHLCSA-N
LogP-3.8
Synonyms
  • Glucose-1-phosphate
  • alpha-D-glucose-1-phosphate
  • cori ester
  • 59-56-3
  • alpha-glucose-1-phosphate
  • Glucose 1-(dihydrogen phosphate)
  • alpha-D-Glucose 1-phosphate
  • alpha-D-Glucopyranosyl phosphate
  • UNII-CIX3U01VAU
  • D-Glucose alpha-1-phosphate
  • D-glucose 1-phosphate
  • 1-O-phosphono-alpha-D-glucopyranose
  • CIX3U01VAU
  • alpha-D-Glucopyranose, 1-(dihydrogen phosphate)
  • CHEBI:29042
  • HXXFSFRBOHSIMQ-VFUOTHLCSA-N
  • Glucose monophosphate
  • glucose-1P
  • alpha-D-glucose-1-P
  • D-glucose-1-phosphate
  • .alpha.-D-Glucopyranose, 1-(dihydrogen phosphate)
  • G1P
  • Glucos P
  • EINECS 200-435-8
  • D-Glucose, 1-(dihydrogen phosphate)
  • alpha-glucose 1-phosphate
  • bmse000086
  • Epitope ID:145002
  • alpha-D-glucopyranose 1-(dihydrogen phosphate)
  • SCHEMBL184242

Applications:

HPLC Separation of Glucose 6- Phosphate and Glucose 1- Phosphate on Newcrom B Column

2020-01-20

Separation type: Liquid Chromatography Mixed-mode

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High Performance Liquid Chromatography (HPLC) Method for Analysis of Glucose 6- Phosphate and Glucose 1- Phosphate

Structural isomer separation poses a challenging task when using reverse-phase chromatography. However both glucose variants, which only differ to which carbon the phosphate group is attached to, can be separated in HPLC isocratically on a reverse-phase mixed-mode anion-exchange Newcrom B column. What differentiates, the Newcrom B phase from other silica-based Analysis of glucose phosphate is important because glucose-6-phosphate is the product of the first step in glycolysis metabolic pathway and glucose-1-phosphate is the first step in glycogenolysis. Glucose-6-phosphate is converted to glucose-1-phosphate and vice versa by the phosphoglucomutase enzyme in biological systems.

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 50/50%
Buffer Formic Acid -  0.5%
Flow Rate 1.0 ml/min
Detection ELSD, 40 °C  MS- compatible mobile phase

Description

Class of Compounds Hydrophilic,  Sugar
Analyzing Compounds Glucose 6- Phosphate,  Glucose 1- Phosphate

Application Analytes:
Glucose 1- Phosphate
Glucose 6-Phosphate