| CAS Number | 59-56-3 |
|---|---|
| Molecular Formula | C6H13O9P |
| Molecular Weight | 230.14 |
| InChI Key | HXXFSFRBOHSIMQ-VFUOTHLCSA-N |
| LogP | -3.8 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Glucose 1-phosphate
January 28, 2026
If you are looking for optimized HPLC method to analyze Glucose 1- Phosphate check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Separation of Glucose 6- Phosphate and Glucose 1- Phosphate on Newcrom B Column
January 20, 2020
HPLC Method for Glucose 6-Phosphate, Glucose 1- Phosphate on Newcrom B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Glucose 6-Phosphate, Glucose 1- Phosphate.
Structural isomer separation poses a challenging task when using reverse-phase chromatography. However both glucose
variants, which only differ to which carbon the phosphate group is attached to, can be separated in HPLC isocratically on a
reverse-phase mixed-mode anion-exchange Newcrom B column.
What differentiates, the Newcrom B phase from other silica-based
Analysis of glucose phosphate is important because glucose-6-phosphate is the product of the first step in glycolysis
metabolic pathway and glucose-1-phosphate is the first step in glycogenolysis. Glucose-6-phosphate is converted to
glucose-1-phosphate and vice versa by the phosphoglucomutase enzyme in biological systems.
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, 40 °C MS- compatible mobile phase |
| Class of Compounds | Hydrophilic, Sugar |
| Analyzing Compounds | Glucose 6-Phosphate, Glucose 1- Phosphate |
Application Column
Newcrom B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Glucose 6-Phosphate
