HPLC Method for DO3A, Tetraxetan (DOTA) on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of DO3A, Tetraxetan (DOTA)
DOTA is a macrocyclic cheating agent with the chemical formula C16H28N4O8. DOTA is actually an acronym for 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid. It is used to bind metal ions, partially due to it’s stability. It is often employed in biomedical applications including but not limited to MRI contrast agents and targeted cancer therapy.
DO3A is a derivative of DOTA with the chemical formula C₁₄H₂₃N₄O₆Na₃.
DO3A, Tetraxetan (DOTA) can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H2SO4 – 0.1 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable, Carboxylic acid, Carbocyclic. |
| Analyzing Compounds | DO3A, Tetraxetan (DOTA) |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Tetraxetan (DOTA)
