HPLC Separation of DOTA and DO3A MS- compatible mobile phase

 HPLC Method for Tetraxetan (DOTA), DO3A on Primesep 100 by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Tetraxetan (DOTA), DO3A 

DOTA is a macrocyclic cheating agent with the chemical formula C16H28N4O8. DOTA is actually an acronym for 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid. It is used to bind metal ions, partially due to it’s stability. It is often employed in biomedical applications including but not limited to MRI contrast agents and targeted cancer therapy.

DO3A is a derivative of DOTA with the chemical formula C₁₄H₂₃N₄O₆Na₃.

Tetraxetan (DOTA), DO3A can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using CAD.

Condition
Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 20/80%
Buffer AmFm pH 3.0- 30 mM
Flow Rate  1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

 

Description
Class of Compounds
 Acid, Hydrophilic, Ionizable, Carboxylic acid, Carbocyclic.
Analyzing Compounds Tetraxetan (DOTA), DO3A

 

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
DO3A
Tetraxetan (DOTA)
Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.