HPLC Separation of DOTA and DO3A MS- compatible mobile phase

 

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer AmFm pH 3.0- 30 mM
Flow Rate  1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase


Description

Class of Compounds
Acid, Hydrophilic, Ionizable, Carboxylic acid, Carbocyclic.
Analyzing Compounds 1,4,7,10-Tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA), DO3A

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
DO3A
Tetraxetan (DOTA)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.