HPLC Separation of DOTA and DO3A on Primesep 100 Column

HPLC Method for DO3A, Tetraxetan (DOTA) on Primesep 100 by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of DO3A, Tetraxetan (DOTA) 

DOTA is a macrocyclic cheating agent with the chemical formula C16H28N4O8. DOTA is actually an acronym for 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid. It is used to bind metal ions, partially due to it’s stability. It is often employed in biomedical applications including but not limited to MRI contrast agents and targeted cancer therapy.

DO3A is a derivative of DOTA with the chemical formula C₁₄H₂₃N₄O₆Na₃.

DO3A, Tetraxetan (DOTA) can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.

Condition

Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 50/50%
Buffer H2SO4 – 0.1 %
Flow Rate 1.0 ml/min
Detection UV, 200 nm

 

Description

Class of Compounds
Acid, Hydrophilic, Ionizable, Carboxylic acid, Carbocyclic.
Analyzing Compounds DO3A, Tetraxetan (DOTA)

 

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
DO3A
Tetraxetan (DOTA)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.