HPLC ELSD Method for Analysis of Diethylentriamine on Primesep 500 Column

HPLC Method for Diethylentriamine on Primesep 500 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Diethylentriamine

Diethylenetriamine, also denoted as dien or DETA, is an organic compound with the chemical formula HN(CH2CH2NH2)2. It is primarily used in as a curing agent for epoxy resins.

Diethylentriamine can be retained and analyzed using the Primesep 500 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid as a buffer. Detection is performed using ELSD.

ColumnPrimesep 500, 2.1 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 10/90 %
BufferAmmonium Acetate pH 3.0 – 20 mM
Flow Rate0.2 ml/min
DetectionELSD
Limit of Detection*1 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Class of Compounds
 Organic Compound
Analyzing CompoundsDiethylentriamine

Application Column

Primesep 500

Column Diameter: 2.1 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Diethylentriamine
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.