HPLC Method for Denatonium benzoate on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Denatonium benzoate
Denatonium benzoate, also known as Denatonium, is the bitterest chemical that is safe to be ingested. It is often used as a bittering agent to antifreeze, detergents, and cosmetics to prevent consumption. Even low concentrations of it can cause am incredibly bitter taste. In France and Italy it is mandatory to add Denatonium to antifreeze. It has the chemical formula C₂₈H₃₄N₂O₃.
Denatonium benzoate can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Denatonium benzoate
Denatonium benzoate, also known as Denatonium, is the bitterest chemical that is safe to be ingested. It is often used as a bittering agent to antifreeze, detergents, and cosmetics to prevent consumption. Even low concentrations of it can cause am incredibly bitter taste. In France and Italy it is mandatory to add Denatonium to antifreeze. It has the chemical formula C₂₈H₃₄N₂O₃.
Benzoic Acid is an organic compound with the chemical formula C7H6O2. It is considered to be the simplest aromatic carboxylic acid. In nature, it can be found in many plants, especially berries. It is used for food preservation of food, as it can inhibit growth of bacteria and mold. While it is safe in food, it can cause irritation to skin, eye, and respiratory systems.
Denatonium benzoate can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Column Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended Mobile Phase Gradient MeCN – 10-90%, 10 min Buffer Gradient H2SO4 – 0.9-0.1%, 10 min Flow Rate 1.0 ml/min Detection UV, 275 nm
Description
Class of Compounds
Drug, The bitter chemical compound, Basic, Hydrophobic, Ionizable Analyzing Compounds Denatonium benzoate
Application Column
Primesep SB
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
