HPLC Separation of Denatonium benzoate on Newcrom A and Primesep SB Column

HPLC.cloud View on hplc.cloud

Condition 1

Column Newcrom А, 3.2x50 mm, 3 µm, 100A
Mobile Phase MeOH/H2O - 20/78.4%
Buffer H2SO4 - 1.6%
Flow Rate 0.5 ml/min
Detection UV 275 nm

Denatonium is the most bitter chemical compound known. Denatonium benzoate is a substance that’s put into household, garden and automotive products to ensure that if a child or animal ever tried to swallow it, they would spit it straight out before they could be poisoned.


Condition 2

Column Primesep SB, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-90%, 10 min
Buffer Gradient H2SO4 - 0.9-0.1%, 10 min
Flow Rate 1.0 ml/min
Detection UV, 275 nm


Class of Compounds Drug, The bitter chemical compound, Basic, Hydrophobic, Ionizable
Analyzing Compounds Denatonium benzoate

Application Analytes:
Benzoic Acid
Denatonium benzoate